protein

Sialoprotein, Bone, Recombinant, Human (BSP)

MBS635865

0.05 mg

740 USD

Recombinant Protein

Sialoprotein, Bone, Recombinant, Human (BSP)

[Sialoprotein, Bone]

[sialoprotein; Bone sialoprotein 2; bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein]

[IBSP; IBSP; BSP; BNSP; SP-II; BSP-II; BNSP; BSP II]

SIAL_HUMAN

Chinese hamster ovary (CHO) cells

>90%, as determined by SDS-PAGE andvisualized by silver stain.

Supplied as a lyophilized powder from20mM MES, 0.5M sodium chloride, pH6.5. Reconstitute with sterile PBS to0.1mg/ml.

Lyophilized powder may be stored at-20 degree C. Stable for 6 months at -20 degree C.Reconstitute with sterile PBS. Aliquot toavoid repeated freezing and thawing. Storeat -20 degree C. For maximum recovery ofproduct, centrifuge the original vial afterthawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant human BSP migrates as an ~75-90kD protein in SDS-PAGE under reducing conditions.

Endotoxin: <1.0EU/ug (LAL).

Activity: Measured by the ability of the immobilizedprotein to support the adhesion of MCF-7cells. When 5x10e4 MCF-7 cells/well areadded to recombinant human IBSP-coatedplates (3ug/ml with 100ul/well),~60-80% will adhere after 30 minutes at 37 degree C. Optimal concentration depends on cell type. Molecular Mass: Recombinant human BSP migrates as an ~75-90kD protein in SDS-PAGE under reducing conditions.

Molecular Biology; MB-Extracellular Matrix Proteins

IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55-75kD, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16aa signal sequence and a 301aa mature region. BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion. Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells.

338084

AAC95490.1

P21815

34.7Kd

ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (106110); Interleukin-11 Signaling Pathway (698753); Osteoblast Signaling Pathway (198842); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979)

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. Protein type: Secreted; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q21.1. Cellular Component: extracellular space; membrane; extracellular region. Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion

0.05 mg

740 USD