product summary
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company name :
MyBioSource
product type :
protein
product name :
LIM Kinase 1, active, Recombinant
catalog :
MBS635863
quantity :
0.005 mg
price :
545 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS635863
products type :
Recombinant Protein
products full name :
LIM Kinase 1, active, Recombinant
products short name :
LIM Kinase 1, active
other names :
LIM domain kinase 1; LIM domain kinase 1; dLIMK
other gene names :
LIMK1; LIMK; LIMK-1
uniprot entry name :
LIMK1_DROME
host :
Recombinant protein expressed in Sf21 cells.
purity :
>90% by SDS-PAGE and Coomassie blue staining.
form :
Supplied as a liquid in 50mM Tris HCl pH 7.5, 150mM sodium chloride, 0.1 mM EGTA, 0.1% b-mercaptoethanol, 0.03% Brij-35, 1 mM benzamidine, 0.2mM PMSF, 50% glycerol.
concentration :
0.3mg/ml
storage stability :
Stable for 6 months at 20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated thawing and freezing.
other info1 :
Specific Activity: 130U/mg. MS Tryptic Fingerprint: Confirmed identity as LIM Kinase 1 with 28% amino acid coverage of the translated sequence listed. Kinase Assay: ~50-400ng phosphorylates 10 ug Cofilin 2 in vitro in the assay described.
other info2 :
Unit Definition: 1 Unit is equal to 1nmol phosphate incorporated into 10ug Cofilin 2 per minute at 30 degree C with a final ATP concentration of 100uM.
products categories :
Molecular Biology; MB-Protein Kinases
products description :
His tagged mouse LIM Kinase 1, with an amino acid 1-14 deletion.
ncbi gi num :
307177067
ncbi acc num :
EFN66335.1
uniprot acc num :
Q8IR79
ncbi mol weight :
72kD
uniprot summary :
Function: Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor tsr/cofilin, thereby stabilizing the actin cytoskeleton. Modulation of actin cytoskeleton dynamics may be essential for imaginal disk morphogenesis and axon guidance. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Subunit structure: Interacts with LATS1, and this interaction inhibits phosphorylation of tsr/cofilin. Ref.6. Subcellular location: Cytoplasm. Cleavage furrow. Midbody. Note: Localizes to the cleavage furrow and the midbody at cytokinesis. Ref.1 Ref.6. Tissue specificity: Expressed throughout the imaginal disks of the eye, leg and wing. Ref.5. Developmental stage: Expressed from early third instar to late pupal stages. Ref.1 Ref.5. Post-translational modification: Phosphorylated on serine and/or threonine residues by ROCK1. Phosphorylated by PAK4 resulting in increased LIMK1 ability to phosphorylate cofilin. May be dephosphorylated and inactivated by SSH1 . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.Contains 2 LIM zinc-binding domains.Contains 1 PDZ (DHR) domain.Contains 1 protein kinase domain.
size1 :
0.005 mg
price1 :
545 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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