protein

Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

MBS635769

0.05 mg

650 USD

Recombinant Protein

Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

Klotho beta

klotho beta; Beta-klotho; beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein; klotho beta; Klotho beta-like protein

KLB; KLB; BKL; BKL; BetaKlotho

KLOTB_HUMAN

Highly Purified. >95% by SDS-PAGE, reducing conditions and visualized with silver stain.

Supplied as a liquid in PBS, pH 6.8, 0.1mM EDTA, 50% glycerol.

1.06mg/ml

Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

SDS-PAGE: 126kD, reducing conditions

Activity: Measured by its binding ability in a functional ELISA. When 2ug/ml (100ul/well) of rhKlotho beta was immobilized onto a Ms x hKlotho beta coated plate, the concentration of rhFGF-21 that produces 50% of the optimal binding response was found to be approximately 3-18ng/ml. Endotoxin Level: <1EU/1ug (LAL)

N-terminal Sequence Analysis: Phe53

Molecular Biology; MB-Enzymes, Glycosidase

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin dependent glucose uptake. The 1043aa type I transmembrane protein is composed of a 51aa signal sequence, a 943aa extracellular domain (ECD) containing two glycosidase-like regions, a 21aa transmembrane domain, and 28aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible. The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD. Murine myeloma cell line, NS0-derived Phe53-Leu997, with a C-terminal 10-His tag.

119613331

EAW92925.1

Q86Z14

110kD

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signal Transduction Pathway (106385); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989); FGFR Ligand Binding And Activation Pathway (106344)

Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: plasma membrane; integral to membrane. Molecular Function: protein binding; fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds. Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response

0.05 mg

650 USD