Mitochondrial glycerol-3-phosphate dehydrogenase, Recombinant, Human (mGPD) | MyBioSource MBS635756 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Mitochondrial glycerol-3-phosphate dehydrogenase, Recombinant, Human (mGPD)

catalog :

MBS635756

quantity :

0.01 mg

price :

400 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS635756

products type :

Recombinant Protein

products full name :

Mitochondrial glycerol-3-phosphate dehydrogenase, Recombinant, Human (mGPD)

products short name :

Mitochondrial glycerol-3-phosphate dehydrogenase

other names :

mitochondrial glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate dehydrogenase, mitochondrial; glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; mtGPD; GPDH-M; mitochondrial glycerophosphate dehydrogenase; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); mtGPD

other gene names :

GPD2; GPD2; GDH2; GPDM; mGPDH; GPD-M; GPDH-M

uniprot entry name :

GPDM_HUMAN

host :

Human, Recombinant, E Coli

purity :

Highly Purified. Purified. 95% by SDS-PAGE.

form :

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM EDTA, 50% glycerol.

concentration :

0.095mg/ml

storage stability :

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA)

app notes :

Suitable for use in ELISA., , Recommended Dilution:, Optimal dilutions to be determined by the researcher.

products categories :

Molecular Biology; MB-Enzymes, Dehydrogenase

products description :

Recombinant mGPD comprises a 558 amino acid fragment (43-600) corresponding to the GlpA domain fragment of the mature mGPD protein and is expressed in E. coli with an amino-terminal hexahistidine tag. Mitochondrial glycerol-3-phosphate dehydrogenase (mGPD) is a Ca2+-sensitive, FAD-binding protein, located on the outer surface of the inner mitochondrial membrane. mGPD catalyses the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Two isoforms have been described for mGPD. Isoform 1 comprises 727aa residues, whereas isoform 2 lacks 126aa residues of the N- terminus.Deficiency of mGPD appears to contribute to the impairment of glucose-stimulated insulin release in several animal models of non-insulin depend diabetes mellitus.

ncbi gi num :

533693

ncbi acc num :

AAA65701.1

uniprot acc num :

P43304

ncbi mol weight :

66.26kD

ncbi pathways :

CDP-diacylglycerol Biosynthesis I Pathway (142255); Fatty Acid Beta Oxidation Pathway (198865); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Glycerol-3-phosphate Shuttle Pathway (545367); Glycerol-3-phosphate Shuttle Pathway (139739); Phosphatidylglycerol Biosynthesis II (non-plastidic) Pathway (142264)

ncbi summary :

The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]

uniprot summary :

GPD2: localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]. Protein type: EC 1.1.5.3; Lipid Metabolism - glycerophospholipid; Oxidoreductase; Mitochondrial; Calcium-binding. Chromosomal Location of Human Ortholog: 2q24.1. Cellular Component: glycerol-3-phosphate dehydrogenase complex; mitochondrial inner membrane. Molecular Function: glycerol-3-phosphate dehydrogenase activity; calcium ion binding. Biological Process: glycerol-3-phosphate metabolic process; camera-type eye development; multicellular organism growth; cellular lipid metabolic process; gluconeogenesis; glycerol catabolic process. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

0.01 mg

price1 :

400 USD

more info or order :

MyBioSource product webpage