protein

Glutathione S Transferase, T1-1, Recombinant Human (GST)

MBS635557

0.1 mg

510 USD

Recombinant Protein

Glutathione S Transferase, T1-1, Recombinant Human (GST)

Glutathione S Transferase, T1-1 Human

glutathione S-transferase theta-1; Glutathione S-transferase theta-1; glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1; glutathione S-transferase theta 1; GST class-theta-1; Glutathione transferase T1-1

GSTT1; GSTT1

GSTT1_HUMAN

This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using. CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values.

~95% (SDS-PAGE). Specifications: >95%

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM DTT, 5mM EDTA and 50% glycerol.

~1mg/ml. Specifications: 2.5mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Biological Activity: ~30 Units/mg using spectrophotometric determination of 4- nitrobenzyl chloride conjugation with reduced glutathione (5mM) in 100mM NaPO4 (pH 6.5) at room temperature.

BioSeparation; BioSep-Assay Detection, Tags

167466164

NP_000844.2

NM_000853.2

P30711

~25kD

Aflatoxin B1 Metabolism Pathway (198808); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343)

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq, Jul 2008]

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family. Protein type: Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: cytosol. Molecular Function: glutathione transferase activity; glutathione peroxidase activity. Biological Process: glutathione metabolic process; xenobiotic metabolic process

0.1 mg

510 USD