Pin1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1, PPIase) | MyBioSource MBS635543 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Pin1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1, PPIase)

catalog :

MBS635543

quantity :

0.005 mg

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS635543

products type :

Recombinant Protein

products full name :

Pin1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1, PPIase)

products short name :

Pin1

other names :

Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; rotamase Pin1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1

other gene names :

PIN1; PIN1; DOD; UBL5; PPIase Pin1

uniprot entry name :

PIN1_HUMAN

host :

E Coli

sequence :

MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

purity :

Highly Purified. >95% as determined by SDS-PAGE.

form :

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM DTT, 20% glycerol.

concentration :

1mg/ml

storage stability :

May be stored at 4 degree C for short-term only. For long-term storage, add a carrier protein (0.1% HAS or BSA), aliquots and store at -20 degree C. Aliquots are stable for 6 months after receipt at – 20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

products categories :

Molecular Biology; MB-Enzymes, Isomerase

products description :

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Recombinant Human PPIase produced in E.Coli is a single,non-glycosylated polypeptide chain containing 163 amino acids & having a molecular mass of 18.2kD.

ncbi gi num :

1332710

ncbi acc num :

AAC50492.1

uniprot acc num :

Q13526

ncbi mol weight :

18.2kD

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); C-MYC Pathway (169344); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)

ncbi summary :

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

PIN1: a member of the parvulin family of peptidyl-prolyl isomerases (PPIase), has been implicated in the G2-M transition of the cell cycle. Has two distinct functional domains: an N-terminal WW domain and a C-terminal PPlase domain. Pin1 interacts with a series of mitotic phosphoproteins, including Plk1, cdc25C and cdc27, catalyzing pSer-Pro or pThr/Pro cis/trans isomerizations. Displays a preference for an acidic residue n-terminal to the isomerized proline bond. Protein type: Isomerase; Nuclear receptor co-regulator; EC 5.2.1.8. Chromosomal Location of Human Ortholog: 19p13. Cellular Component: nucleoplasm; cytoplasm; nuclear speck; midbody; nucleus. Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; mitogen-activated protein kinase kinase binding; phosphothreonine binding; GTPase activating protein binding; phosphoserine binding. Biological Process: protein peptidyl-prolyl isomerization; positive regulation of ubiquitin-protein ligase activity; cytokine and chemokine mediated signaling pathway; regulation of mitosis; innate immune response; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle; negative regulation of interferon type I production; regulation of cytokinesis

size1 :

0.005 mg

price1 :

275 USD

size2 :

0.02 mg

price2 :

340

more info or order :

MyBioSource product webpage