protein

Fibronectin, Bovine

MBS635038

0.1 mg

220 USD

Protein

Fibronectin, Bovine

Fibronectin, Bovine

fibronectin; Fibronectin; fibronectin; FN; gene C; fibronectin 1

fn1; fn1; fibronectin; FN

FINC_XENLA

Bovine plasma

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a sterile-filtered solution in 0.4M Urea, 50mM Tris-HCl, pH 7.5, 0.5M sodium chloride.

1.37 mg/ml

Samples are stable for up to six months at 2-8 degree C. Product is not stable at room temperature. Avoid vortexing and excessive agitation. Aliquot into 50-100ul volumes and store at -20 degree C for up to six months in a manual defrost. freezer. Avoid repeated freeze-thaw cycles.

ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)

ELISA, Ouchterlony, Immunoblotting. Used in vitro differentiation of mouse ES cells into neural cells. Suitable for use in mouse embryonic stem cell research. Use in Basal Medium A and as a coating agent for coverslips. This component is recommended for growth and survival of neural cell types during in vitro differentiation of mouse ES cells.

Activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. Endotoxin Level: 1.0 EU per 1 ug of fibronectin as determined by the LAL method.

Recommended Dilution: To coat coverslips, prepare a 1mg/ml solution. For use as a substratum for cell attachment and spreading, use at 1-5ug/cm2. To support cell spreading, add 0.5-50ug/ml in media.

Molecular Biology; MB-Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules.1 It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when on the surface.2 Polymerization of fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), fibronectin plays an important role in normal

148237139

NP_001081270.1

NM_001087801.1

Q91740

220kD

ECM-receptor Interaction Pathway (119288); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (119287); Focal Adhesion Pathway (478); Regulation Of Actin Cytoskeleton Pathway (119294); Regulation Of Actin Cytoskeleton Pathway (500)

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction . By similarity. Subunit structure: Dimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends . By similarity. Tissue specificity: In early Xenopus embryo, cellular forms of fibronectin predominate which include both extra domains. In fibronectin of embryonic and adult liver the connecting strand 3 can be either completely excluded or included. Post-translational modification: Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.

0.1 mg

220 USD

1 mg

405