catalog number :
MBS635008
products full name :
Myelin Basic Protein, Human Brain, lyophilized (MBP)
products short name :
Myelin Basic Protein Brain, lyophilized
form :
Supplied as a lyophilized powder. No preservatives added. Reconstitute with 10mM HCl.
storage stability :
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with with 10mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA)
app notes :
Suitable for use in ELISA or for antigen applications in immunological protocols. Immunoreactivity is verified using polyclonal and monoclonal antibodies against MBP. Other applications not tested.
products categories :
Molecular Biology; MB-Myelin
products description :
In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
ncbi mol weight :
MBP contains 14.2kD (13.0 minor) and 18.5kD (17.2 minor) isoforms as well as their dimers (13.5% SDS-PAGE, non-reducing conditions).