protein

Myelin Basic Protein, Dephosphorylated, Bovine (MBP)

MBS634877

5 mg

520 USD

Protein

Myelin Basic Protein, Dephosphorylated, Bovine (MBP)

Myelin Basic Protein, Dephosphorylated, Bovine

Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1; MAP kinase; mitogen-activated protein kinase 1a; mitogen-activated protein kinase 1

mapk1-a; erk; erk2; ert1; mapk; mpk1; xp42; mapk1; mapk2; prkm1; prkm2; mapk1a; p42mapk

Bovine brain

Highly Purified. Purified from bovine brain using HPLC and de-phosphorylated using Lambda protein phosphatase. Lambda protein phosphatase was inactivated, in the presence of 10mM sodium EDTA, at 65 degree C for one hour. Purity was assessed at 95% using

Supplied as a liquid in 1 OmM MOPS, pH 7.0, 0.246uM MnCI2, 1.23uM EDTA, 385ng inactive lambda phosphatase, 0.05% sodium azide.

5mg/ml

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

Recommended Dilutions: . Western Blot: 1 ug/ml detected MBP when used to probe 800ng MBP incubated with and without MAPK2. Protein Kinase Assay: Dephosphorylated MBP incorporated 32P-phosphate In radioactive kinase reactions using MAPK2/Erk2. Phosphorylated in a non-radioactive kinase assay using M2362-06 (MAP Kinase 2, Recombinant, Mouse). MBP phosphorylation was detected by Western Blot using M9758-05 (Myelin Basic Protein, phosphorylated (MBP)). No detectable endogenous phosphorylation on MBP was seen as determined by Western Blot of 1 ug of the dephosphorylated MBP with 1 ug/ml M9758-05. Phosphatase Acti'/ity: When incubated with pNPP for 45 minutes, 400ug had no detectable levels of active phospnatase. Optimal dilutions to be determined by the researcher.

Molecular Biology; MB-Myelin

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golh aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an Integral part of the Gofli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Developed Tor use in radioactive and non-radioactive kinase assays.

117949815

P26696.3

Adherens Junction Pathway (119290); Adherens Junction Pathway (481); Dorso-ventral Axis Formation Pathway (119286); Dorso-ventral Axis Formation Pathway (472); ErbB Signaling Pathway (84646); ErbB Signaling Pathway (458); Focal Adhesion Pathway (119287); Focal Adhesion Pathway (478); Gap Junction Pathway (119292); Gap Junction Pathway (483)

5 mg

520 USD