protein

Myelin Basic Protein (MBP) (Biotin)

MBS634854

1 mg

590 USD

Protein

Myelin Basic Protein (MBP) (Biotin)

[Myelin Basic Protein]

[myelin basic protein; Myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein]

[MBP; MBP; MBP]

MBP_HUMAN

Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.

3.71 mg/ml

Stable for 6 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Suitable for use in Protein Kinase Assay and radioactive kinase assays. Other applications have not been tested

Testing Data

Western Blot (WB)

Source: Bovine brain. Grade: Highly Purified. Biotin/Protein Molar Ratio: 1.0. Protein Kinase Assay: Phosphorylated in a radioactive kinase assay using MAP Kinase 2, Recombinant , Mouse (MBS637602). 32P-phosphorylated, biotinylated MBP was captured using Streptavidin (Agarose) (MBS634775)

Purity: Purified using FPLC liquid chromatography and dephosphorylated using Lambda protein phosphatase. Lambda protein phosphatase was inactivated, in the presence of 10mM sodium EDTA, at 65°C for one hour. Purity was assessed at 95% using SDS-PAGE and Coomasie blue staining of 1ug of product.

Molecular Biology; MB-Myelin

In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.

1162922

AAC41944.1

P02686

33,117 Da

Glial Cell Differentiation Pathway (698758); MAPK Cascade Pathway (198834); Neural Crest Differentiation Pathway (672460); Spinal Cord Injury Pathway (739007)

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 18q23. Cellular Component: internode region of axon; compact myelin; cell soma; plasma membrane; nucleus. Molecular Function: protein binding; protease binding; structural constituent of myelin sheath. Biological Process: myelination; response to mercury ion; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; immune response; negative regulation of axonogenesis; axon ensheathment; response to progesterone stimulus; aging

1 mg

590 USD