catalog number :
MBS634302
products full name :
Histone H3 (F3), Arginine Rich
products short name :
Histone H3
host :
Bovine tissue (thymus)
specificity :
Bands at 15.3, 14 kD (20%SDS-PAGE)
form :
Supplied as a white powder. Reconstitute with cold, sterile 0.9M acetic acid to ~1mg/ml. Prepare fresh solution before use.
storage stability :
Store lyophilized histones desiccated at 4 degree C. Reconstitute per reconstitution instructions. Do not store reconstituted histones. Histones lose activity in solutions. Further dilutions can be made in assay buffer.
other info1 :
Country of Origin: USA
products categories :
Molecular Biology; MB-Histones
products description :
Histone subclass H3(F3), may also contain H2b(F2b) and H2a(F2a2). Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. ThiS allows for the supercoiling of the chromatin fibers. With the exception of H 1 , the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly - conserved (low mutation rate) proteins in nature. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The hlstones are a group of water and dilute acid soluble l5asic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limitednumber of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamiC such that the histones of a single cell type are found to vary during development. They are believed to playa role in gene activity and cellular metabolism.
ncbi acc num :
BAA31218.1
ncbi mol weight :
Molecular Weights of Histones: Lysine Rich (h1, F1): ~21,500; Slightly Lysine Rich (H2a, F2a2): 14,004; Slightly Lysine Rich (H2b; F2b): 13,774; Arginine Rich (H3,F3): 15,324; Argine Rich (H4, F2a1): 11,282