product summary
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company name :
MyBioSource
product type :
antibody
product name :
BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC)
catalog :
MBS633646
quantity :
120 Tests
price :
640 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
[11K3]
application :
ELISA, flow cytometry, FACS, enzyme immunoassay
more info or order :
image
image 1 :
Binding of MBS633646 to human PBL
product information
catalog number :
MBS633646
products type :
Antibody
products full name :
BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC)
products short name :
[BTLA]
products name syn :
[Anti -BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (FITC)]
other names :
[BtlA]
clonality :
Monoclonal
isotype :
IgG1,k
clone :
[11K3]
host :
Mouse
reactivity :
Human
specificity :
Recognizes human CD272 (BTLA). Binds to CD272 on the cell surface of human PBMC in Flow Cytometry and to recombinant CD272-muIg in ELISA. Does not block binding of Biotinylated CD270 (HVEM)-muIg to CD272-muIg in ELISA, but appears to enhance this interaction.
purity :
Affinity Purified. Purified by Protein A affinity chromatography.
form :
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
concentration :
0.25 mg/ml
storage stability :
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
tested application :
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
app notes :
Suitable for use in ELISA and Flow Cytometry. Dilution: ELISA: Detection. Flow Cytometry: 1:50. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml, after which they were washed and incubated 45 minutes on ice with 80ul of B5001-61K at a 1:50 dilution (5ug/ml). 2. They were then washed three times, fixed and analyzed by FACS using a lymphoid gate. 3. Cells stained positive with a mean shift of 0.54 log10 fluorescent units when compared to a Mouse IgG1 (FITC) negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of B5001-61G.
image1 heading :
Testing Data
other info1 :
Immunogen: Recombinant protein corresponding to soluble human CD272
products categories :
Antibodies; Abs to CD Markers
products description :
Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines. Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis.
ncbi gi num :
25140977
ncbi acc num :
AAN71743.1
size1 :
120 Tests
price1 :
640 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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