CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G Protein-coupled Receptor 2, GRP2) (APC) | MyBioSource MBS633558 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G Protein-coupled Receptor 2, GRP2) (APC)

catalog :

MBS633558

quantity :

100 Tests

price :

835 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

[7H55]

reactivity :

human, mouse, rat

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

A) Mouse B16F1 cells were stained with MBS633558 (filled histogram) or APC-conjugated isotype control (open histogram).

image 2 :

B) Rat splenocytes were stained with MBS633558 and PE-conjugated anti-rat CD3. Quadrant markers were set based upon isotype control staining.

product information

catalog number :

MBS633558

products type :

Antibody

products full name :

CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G Protein-coupled Receptor 2, GRP2) (APC)

products short name :

[CCR10]

products name syn :

[Anti -CCR10 (CC Chemokine Receptor 10, CC-CCR-10, G Protein-coupled Receptor 2, GRP2) (APC)]

other names :

[C-C chemokine receptor type 10; C-C chemokine receptor type 10; C-C chemokine receptor type 10; CCR-10; CC-CKR-10; C-C CKR-10; OTTHUMP00000163143; CC chemokine receptor 10; G protein-coupled receptor 2; G-protein coupled receptor 2; chemokine (C-C motif) receptor 10; G-protein coupled receptor 2]

other gene names :

[CCR10; CCR10; GPR2; GPR2]

uniprot entry name :

CCR10_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[7H55]

host :

Rat

reactivity :

Mouse, Rat

specificity :

Recognizes mouse CCR10. Species Crossreactivity: rat

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

concentration :

0.01 mg/ml

storage stability :

May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.

tested application :

Flow Cytometry (FC/FACS)

app notes :

Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Neat; 10ul labels 1x10e6 cells. Validated for use with mouse B16F1 cells and rat splenocytes. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse or rat IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of C2100-16A to up to 1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final analysis by flow cytometry. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2B antibody.

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Immunogen: C6 cells transfected with mouse CCR10 (aa1-362)

products categories :

Antibodies; Abs to G Protein Receptors

products description :

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets, and functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

ncbi gi num :

156104886

ncbi acc num :

NP_057686.2

ncbi gb acc num :

NM_016602.2

uniprot acc num :

P46092

ncbi mol weight :

38,416 Da

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

ncbi summary :

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM]

uniprot summary :

CCR10: Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 17q21.1-q21.3. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis

size1 :

100 Tests

price1 :

835 USD

more info or order :

MyBioSource product webpage