Actin, alpha, Smooth Muscle (PE) | MyBioSource MBS633258 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Actin, alpha, Smooth Muscle (PE)

catalog :

MBS633258

quantity :

100 Tests

price :

710 USD

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

[9H15]

reactivity :

human, mouse, rat, dog, chicken, cow

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Intracellular staining of the mouse fibroblast cell line L929 with MBS633258 (filled histogram) or with isotype control (open histogram).

product information

catalog number :

MBS633258

products type :

Antibody

products full name :

Actin, alpha, Smooth Muscle (PE)

products short name :

[Actin, alpha, Smooth Muscle]

products name syn :

[Anti -Actin, alpha, Smooth Muscle (PE)]

other names :

[actin, alpha 2, smooth muscle, aorta, isoform CRA_a; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein]

other gene names :

[ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS]

uniprot entry name :

ACTA_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[9H15]

host :

Mouse

reactivity :

Bovine, Canine, Chicken, Frog, Goat, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep

specificity :

Recognizes human alpha-Smooth Muscle Actin. Species Crossreactivity: bovine, chicken, frog, goat, guinea pig, mouse, rabbit, rat, canine, sheep and snake.

purity :

Affinity Purified. Purified by Protein G affinity chromatography

form :

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

storage stability :

4 degree C Do not freeze

tested application :

Flow Cytometry (FC/FACS)

app notes :

Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Neat. 10ul labels up to 1x10e6 cells.

image1 heading :

Testing Data

other info1 :

Immunogen: Synthetic decapeptide corresponding to the N-terminal residues of human alpha-Smooth Muscle Actin.

other info2 :

Hybridoma: Fusion of a mouse myeloma with B cells obtained from a rat immunized with a synthetic decapeptide from the N-terminal of Alpha-Smooth Muscle Actin. Storage and Stability:. Maybe stored at 4 degree C before opening. DO NOT FREEZE! Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

products categories :

Antibodies; Abs to Actin Related Proteins

products description :

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.

ncbi gi num :

119570540

ncbi acc num :

EAW50155.1

uniprot acc num :

P62736

ncbi mol weight :

42,009 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Myometrial Relaxation And Contraction Pathways (198759); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (198903); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

uniprot summary :

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: protein kinase binding; ATP binding. Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction. Disease: Multisystemic Smooth Muscle Dysfunction Syndrome; Moyamoya Disease 5; Aortic Aneurysm, Familial Thoracic 6

size1 :

100 Tests

price1 :

710 USD

more info or order :

MyBioSource product webpage