FceRI, alpha (Fc epsilon RI, IgE Receptor) | MyBioSource MBS632728 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

FceRI, alpha (Fc epsilon RI, IgE Receptor)

catalog :

MBS632728

quantity :

0.2 mg

price :

630 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[0.T.195]

reactivity :

human

application :

western blot, immunoprecipitation, FACS, other

more info or order :

MyBioSource product webpage

image

image 1 :

IP/WB: Biotinylated rat basophilic leukemic cell lysate was incubated with MBS632728 (5ug).

product information

catalog number :

MBS632728

products type :

Antibody

products full name :

FceRI, alpha (Fc epsilon RI, IgE Receptor)

products short name :

[FceRI, alpha]

products name syn :

[Anti -FceRI, alpha (Fc epsilon RI, IgE Receptor)]

other names :

[Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; High affinity immunoglobulin epsilon receptor subunit alpha; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc-epsilon RI-alpha; FcERI; IgE Fc receptor subunit alpha]

other gene names :

[FCER1A; FCER1A; FCE1A; FcERI; FCE1A; FcERI]

uniprot entry name :

FCERA_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[0.T.195]

host :

Mouse

specificity :

Recognizes rat FceRI alpha at ~45-55kD.

purity :

Affinity Purified. Purified by Protein G affinity chromatography.

form :

Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

concentration :

1 mg/mL

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in Immunoprecipitation/Western Blot and FACS. Not suitable for Western Blot without pre-immunoprecipitation of antibody. Other applications have not been tested.

app notes :

Immunoprecipitation: 5 ug immunoprecipitates rat FceRI alpha from 500 ug of biotinylated rat basophilic leukemia cell lysate. The immunoprecipitate was resolved by electrophoresis and transferred to nitrocellulose. Biotinylated protein bands wew visualized using NeutrAvidin labeled with HRP and and enhanced chemiluminesce detection system. Optimal dilutions to be determined by the researcher.

image1 heading :

Testing Data

other info1 :

Immunogen: Whole rat basophilic leukemia cells, positive for the rat high-affinity receptor recognizing immunoglobulin E (FceRI).

products categories :

Antibodies; Abs to Receptors

products description :

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE-FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of an a-chain, a b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

ncbi gi num :

15929530

ncbi acc num :

AAH15195.1

uniprot acc num :

P12319

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825); Fc-epsilon Receptor I Signaling In Mast Cells Pathway (138050); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

uniprot summary :

Function: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. Subunit structure: Tetramer of an alpha chain, a beta chain, and two disulfide linked gamma chains. Subcellular location: Cell membrane; Single-pass type I membrane protein. Sequence similarities: Contains 2 Ig-like (immunoglobulin-like) domains.

size1 :

0.2 mg

price1 :

630 USD

more info or order :

MyBioSource product webpage