TSTA3 (Tissue Specific Transplantation Antigen P35B, Protein FX, GDP-L-fucose Synthase, Red Cell NADP(H)-binding Protein, Short-chain Dehydrogenase/Reductase Family 4E Member 1, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase) | MyBioSource MBS632306 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TSTA3 (Tissue Specific Transplantation Antigen P35B, Protein FX, GDP-L-fucose Synthase, Red Cell NADP(H)-binding Protein, Short-chain Dehydrogenase/Reductase Family 4E Member 1, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase)

catalog :

MBS632306

quantity :

0.1 mg

price :

580 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2B9]

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of TSTA3 expression in HeLa, using MBS632306.

image 2 :

Detection limit for recombinant GST tagged TSTA3 is 0.3 ng/ml as a capture antibody.

product information

catalog number :

MBS632306

products type :

Antibody

products full name :

products short name :

[TSTA3]

products name syn :

[Anti -TSTA3 (Tissue Specific Transplantation Antigen P35B, Protein FX, GDP-L-fucose Synthase, Red Cell NADP(H)-binding Protein, Short-chain Dehydrogenase/Reductase Family 4E Member 1, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase)]

other names :

[TSTA3 protein; GDP-L-fucose synthase; GDP-L-fucose synthase; OTTHUMP00000228670; OTTHUMP00000228671; OTTHUMP00000228672; 3-5 epimerase/4-reductase; red cell NADP(H)-binding protein; tissue specific transplantation antigen 3; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; short chain dehydrogenase/reductase family 4E, member 1; tissue specific transplantation antigen P35B; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; Protein FX; Red cell NADP(H)-binding protein; Short-chain dehydrogenase/reductase family 4E member 1]

other gene names :

[TSTA3; TSTA3; FX; P35B; SDR4E1; SDR4E1]

uniprot entry name :

FCL_HUMAN

clonality :

Monoclonal

isotype :

IgG3, k

clone :

[2B9]

host :

Mouse

reactivity :

Human

specificity :

Recognizes human TSTA3.

purity :

Purified. Purified.

form :

Supplied as a liquid in PBS, pH 7.4.

concentration :

0.5mg/ml

storage stability :

May be stored at 4 degree C for short-term only.For long term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB)

app notes :

Suitable for use in ELISA and Western Blot. Optiminal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Testing Data

image4 heading :

Western Blot (WB)

image4 description :

Western Blot analysis of TSTA3 over-expressed 293 cell line, cotransfected with TSTA3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MBS632306. GAPDH (36.1kD) used as specificity and loading control.

other info1 :

Immunogen: TSTA3 (NP_003304, 222-322aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.

products categories :

Antibodies; Abs to Enzymes, Synthase

products description :

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.

ncbi gi num :

62204882

uniprot acc num :

Q13630

ncbi mol weight :

35,893 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Metabolic Pathways (132956)

ncbi summary :

uniprot summary :

TSTA3: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. Belongs to the fucose synthase family. Protein type: Oxidoreductase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 1.1.1.271; Carbohydrate Metabolism - fructose and mannose. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytoplasm. Molecular Function: GDP-L-fucose synthase activity; electron carrier activity; isomerase activity; coenzyme binding; GDP-4-dehydro-D-rhamnose reductase activity. Biological Process: leukocyte adhesion; cytolysis; GDP-mannose metabolic process; de novo GDP-L-fucose biosynthetic process

size1 :

0.1 mg

price1 :

580 USD

more info or order :

MyBioSource product webpage