antibody

Urokinase Plasminogen Activator Receptor (U Plasminogen Activator Receptor, UPA Receptor, uPAR, U-PAR, CD87, CD87 Antigen, MO3, Monocyte Activation Antigen Mo3, Plasminogen Activator Urokinase Receptor, PLAUR, URKR) (PE)

MBS632008

100 Tests

670 USD

monoclonal

rat

PE

[10K37]

human, mouse

western blot, flow cytometry, other

Antibody

Urokinase Plasminogen Activator Receptor (U Plasminogen Activator Receptor, UPA Receptor, uPAR, U-PAR, CD87, CD87 Antigen, MO3, Monocyte Activation Antigen Mo3, Plasminogen Activator Urokinase Receptor, PLAUR, URKR) (PE)

[Urokinase Plasminogen Activator Receptor]

[Anti -Urokinase Plasminogen Activator Receptor (U Plasminogen Activator Receptor, UPA Receptor, uPAR, U-PAR, CD87, CD87 Antigen, MO3, Monocyte Activation Antigen Mo3, Plasminogen Activator Urokinase Receptor, PLAUR, URKR) (PE)]

[urokinase plasminogen activator receptor; Urokinase plasminogen activator surface receptor; urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; plasminogen activator, urokinase receptor; Monocyte activation antigen Mo3]

[PLAUR; PLAUR; CD87; UPAR; URKR; U-PAR; MO3; UPAR]

UPAR_HUMAN

Monoclonal

IgG2A

[10K37]

Rat

Mouse

Recognizes mouse uPAR. No crossreactvity with recombinant human uPAR in Western Blot.

Purified by Protein G affinity chromatography.

Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

0.025 mg/ml

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot and Flow Cytometry. Other applications not tested.

Flow Cytometry: 10ul/10e6 cells using mouse whole blood monocytes. Optimal dilutions to be determined by the researcher.

Flow Cytometry (FC/FACS)

Immunogen: Recombinant protein corresponding to Leu24-Thr297 from mouse uPAR expressed in NS0 cells. UniProt Accession: P35456.

Hybridoma: Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse uPAR

Antibodies; Abs to Cancer Markers

CD87, a 45- to 60kD protein, consists of three structurally homologous domains of ~90aa, designated D1, D2, and D3, each containing a conserved arrangement of disulfide bonds and separated by short interdomain linker sequences. uPAR exists in various molecular forms; heavily glycosylated, a cleaved form, a soluble form and spliced forms. CD87 is expressed by monocytes/macrophages, neutrophils, endothelial, smooth muscle cells and epithelial cells. CD87 is overexpressed upon exposure to inflammatory mediators. The protein is also highly expressed by tumor cells and contributes to their invasiveness and metastatic potential. CD87 is a multiligand receptor that operates as a key element in pathophysiological processes involving cell migration and tissue remodelling during inflammation and cancer metastasis. CD87 binds with high affinity the Ser-proteinase urokinase-type plasminogen activator (uPA) which, upon binding catalyzes the conversion of plasminogen into plasmin which activates various matrix metalloproteinase leading to a high potential for pericellular proteolysis. CD87 functions also as an adhesion molecule by binding vitronectin, thus contributing to leukocytic adherence. High-affinity binding of CD87 to both uPA and vitronectin requires the presence of domain 1 (D1). Yet, CD87 participates in cell adherence and migration also through two other routes, a physical association and functional interaction with various integrins and via an intrinsic chemotactic activity associated to the D1-D2 linker sequence.

433901

Q03405

36,978 Da

Arf6 Downstream Pathway (138038); Attachment Of GPI Anchor To UPAR Pathway (106239); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); FGF Signaling Pathway (137989); Hemostasis Pathway (106028); Metabolism Of Proteins Pathway (106230); Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway (106236)

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq]

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 19q13. Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; endoplasmic reticulum lumen; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor activity; receptor binding. Biological Process: fibrinolysis; cellular protein metabolic process; attachment of GPI anchor to protein; regulation of proteolysis; C-terminal protein lipidation; blood coagulation; post-translational protein modification; signal transduction; chemotaxis; cell motility

100 Tests

670 USD