catalog number :
MBS631901
products full name :
Microcystin LR
products short name :
Microcystin LR
products name syn :
Anti -Microcystin LR
other names :
Microcystin LR degradation protein MlrC
specificity :
Recognizes Microcystin-LR.
purity :
Affinity Purified. Purified by Protein G affinity chromatography.
form :
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
ELISA (EL/EIA)
app notes :
Suitable for use in ELISA.
other info1 :
Immunogen: Microcystin-LR-BSA conjugate.
other info2 :
Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice.
products categories :
Antibodies; Abs to Toxins
products description :
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition (-D-Ala-L-X-D-erythro-b-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 amino acid 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-amino acids.With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
ncbi acc num :
AEL26184.1