antibody

NME1 (Nucleoside Diphosphate Kinase A, NDP Kinase A, NDK A, Tumor Metastatic Process-Associated Protein, Metastasis Inhibition Factor nm23, nm23-H1, Granzyme A-Activated DNase, GAAD, NDPKA, NM23) (Azide Free)

MBS631838

0.1 mg

515 USD

monoclonal

mouse

nonconjugated

11C503

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

NME1 (Nucleoside Diphosphate Kinase A, NDP Kinase A, NDK A, Tumor Metastatic Process-Associated Protein, Metastasis Inhibition Factor nm23, nm23-H1, Granzyme A-Activated DNase, GAAD, NDPKA, NM23) (Azide Free)

NME1

Anti -NME1 (Nucleoside Diphosphate Kinase A, NDP Kinase A, NDK A, Tumor Metastatic Process-Associated Protein, Metastasis Inhibition Factor nm23, nm23-H1, Granzyme A-Activated DNase, GAAD, NDPKA, NM23) (Azide Free)

nucleoside diphosphate kinase A isoform a; Nucleoside diphosphate kinase A; nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in; NME/NM23 nucleoside diphosphate kinase 1; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; nm23-H1

NME1; NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; NDPKA; NM23; NDK A; NDP kinase A; GAAD

NDKA_HUMAN

Monoclonal

IgG1,k

11C503

Mouse

Human

Recognizes human NME1.

Purified. Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)

Suitable for use in ELISA, Immunofluorescence, and Western Blot. Dilution: ELISA: 1ug/ml-3ng/ml. Western Blot: 1:500-1:1000

Immunogen: Full length recombinant protein corresponding to aa1-153 of human NME1 with GST tag. MW of the GST tag alone is ~26kD.

Antibodies; Abs to Protein Kinases

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

38045913

NP_937818.1

NM_198175.1

P15531

17,149 Da

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Arf6 Downstream Pathway (138038); Arf6 Trafficking Events Pathway (137954); CMP Phosphorylation Pathway (782393); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (618581); Guanine Ribonucleotide Biosynthesis IMP = GDP,GTP Pathway (468243); Integrated Pancreatic Cancer Pathway (711360); Metabolism Pathway (477135)

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3 -5 exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3 end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported. Protein type: EC 2.7.4.6; Kinase, other; Tumor suppressor; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; Protein kinase, histidine; Other group; NDK family. Chromosomal Location of Human Ortholog: 17q21.3. Cellular Component: membrane; mitochondrion; cytoplasm; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; GTP binding; magnesium ion binding; nucleoside diphosphate kinase activity; deoxyribonuclease activity; ribosomal small subunit binding; ATP binding. Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding. Disease: Neuroblastoma, Susceptibility To

0.1 mg

515 USD