Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase) | MyBioSource MBS631534 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

catalog :

MBS631534

quantity :

0.1 mL

price :

600 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[5i133]

reactivity :

human, mouse

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or lambda phosphatase-treated, using MBS631534 (upper) and V2110-18C (lower).

image 2 :

Western Blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using MBS631534 (upper) or T9228-75B (lower). CKR/PAE cells (lanes 13, 14) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain.

image 3 :

Immunohistochemical analysis of paraffin-embedded HUVE cells, untreated (left) or VEGF-treated (right), using MBS631534.

product information

catalog number :

MBS631534

products type :

Antibody

products full name :

Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

products short name :

[Vascular Endothelial Growth Factor Receptor 2]

products name syn :

[Anti -Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr951) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)]

other names :

[vascular endothelial growth factor receptor 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor 2; soluble VEGFR2; OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1]

other gene names :

[KDR; KDR; FLK1; CD309; VEGFR; VEGFR2; FLK1]

uniprot entry name :

VGFR2_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[5i133]

host :

Rabbit

reactivity :

Human, Mouse

specificity :

Recognizes human VEGF receptor 2 only when phosphorylated at Tyr951 at ~230kD. May crossreact with activated VEGF receptor 1, but does not react with other related tyrosine phosphorylated tyrosine kinases. Species Crossreactivity: mouse

purity :

Purified

form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in ELISA (EIA), Western Blot (WB), and Immunohistochemistry (IHC). Other applications not tested.

app notes :

Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking , overnigth. Immunohistochemistry(Paraffin): 1:125 Requires HIER using EDTA, pH 8.0 prior to staining and TBST-5% NGS as antibody diluent. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2 (KLH). Grade: Purified

products categories :

Antibodies; Abs to Receptors

products description :

Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2. The phosphorylation of Tyr1212 provides a docking site for Grb2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCg, as well as Shb. Signaling from VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo.

ncbi gi num :

11321597

ncbi acc num :

NP_002244.1

ncbi gb acc num :

NM_002253.2

uniprot acc num :

P35968

ncbi pathways :

Angiogenesis Pathway (198772); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); HIF-2-alpha Transcription Factor Network Pathway (137956); Id Signaling Pathway (198871)

ncbi summary :

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq]

uniprot summary :

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability. Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; VEGFR family. Chromosomal Location of Human Ortholog: 4q11-q12. Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; plasma membrane; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane. Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; Hsp90 protein binding; ATP binding. Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of focal adhesion formation; positive regulation of MAPKKK cascade; ovarian follicle development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis. Disease: Hemangioma, Capillary Infantile

size1 :

0.1 mL

price1 :

600 USD

more info or order :

MyBioSource product webpage