anti-Human NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) | MyBioSource MBS631230 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

anti-Human NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)

catalog :

MBS631230

quantity :

0.1 mL

price :

420 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human placenta stained with MBS631230 using peroxidase-conjugate and AEC chromogen. Cytoplasmic staining of trophoblasts, fibroblasts and endothelial cells can be seen.

image 2 :

Western Blot using MBS631230.

product information

catalog number :

MBS631230

products type :

Antibody

products full name :

anti-Human NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)

products short name :

[NFkB, p65]

products name syn :

[Anti -NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)]

other names :

[NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c; NF-kappa-B inhibitor-interacting Ras-like protein 2; NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like 2; I-kappa-B-interacting Ras-like protein 2]

other gene names :

[NKIRAS2; NKIRAS2; KBRAS2; kappaB-Ras2; KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2]

uniprot entry name :

KBRS2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Canine, Drosophila, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Xenopus

specificity :

Recognizes human NF kappa B at ~65kD. Species Crossreactivity: mouse, rat, hamster, rabbit, bovine, porcine, canine, Drosophila and Xenopus.

purity :

Affinity Purified. Purified by Protein A affinity chromatography.

form :

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

concentration :

1mg/ml

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Suitable for use in Western Blot, ELISA and Immunohistochemistry. Dilution: Western Blot: 5-10ug/ml for 2 hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide from the C-terminus of human NF kappa B protein. Cellular Localization: Cytoplasmic.

other info2 :

Positive Control: Jurkat cells, Placenta

products categories :

Antibodies; Abs to Transcription Factors

products description :

NF kappa B is a heterodimer consisting of a 50kD DNA binding subunit and a 65kD transactivating subunit (RelA). The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IkB. Upon stimulation of cells with stimuli like LPS, Interleukin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression. NFkB was originally identified as transcriptional activator for kappa light cahain gene in B cells.

ncbi gi num :

222136632

ncbi acc num :

NP_001138401.1

ncbi gb acc num :

NM_001144929.1

uniprot acc num :

Q9NYR9

ncbi mol weight :

21,508 Da

ncbi pathways :

TNF-alpha/NF-kB Signaling Pathway (198884)

uniprot summary :

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: G protein, monomeric, Ras; G protein; G protein, monomeric. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: membrane; cytoplasm. Molecular Function: GTPase activity; GTP binding. Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction

size1 :

0.1 mL

price1 :

420 USD

size2 :

0.25 mL

price2 :

470

more info or order :

MyBioSource product webpage