antibody

Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

MBS630904

0.1 mL

465 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

Insulin-Like Growth Factor I Receptor, beta

Anti -Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor

IGF1R; IGF1R; IGFR; CD221; IGFIR; JTK13; IGF-I receptor

IGF1R_HUMAN

Polyclonal

IgG

Rabbit

Human, Monkey, Mouse, Rat

Recognizes endogenous levels of human Insulin-Like Growth Factor I Receptor beta at ~95kD. Does not crossreact with insulin receptor. Species Crossreactivity: mouse, rat and monkey.

Affinity Purified. Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Dilution: Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:2400. Use citrate as unmasking buffer. Immunoprecipitation: 1:100

Immunogen: Synthetic peptide corresponding to the carboxy-terminal residues of human IGF1Rb.

Antibodies; Abs to Receptors

Type I insulin-like growth factor receptor (IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines (1-3). Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant similarity with IGF-I receptors in both structure and function. There is also an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151). Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation (8).

4557665

NP_000866.1

NM_000875.3

P08069

154,793 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Apoptosis Pathway (198797); Endochondral Ossification Pathway (198812); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Glioma Pathway (83110)

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008]

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541. Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; TK group; InsR family. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex. Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; insulin receptor substrate binding; protein-tyrosine kinase activity; G-protein alpha-subunit binding; phosphoinositide 3-kinase binding; ATP binding; insulin receptor binding; insulin-like growth factor receptor activity. Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; mammary gland development; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; insulin-like growth factor receptor signaling pathway; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis. Disease: Insulin-like Growth Factor I, Resistance To

0.1 mL

465 USD