c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR) | MyBioSource MBS629762 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

catalog :

MBS629762

quantity :

0.05 mg

price :

670 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse, dog

application :

immunohistochemistry, flow cytometry

more info or order :

MyBioSource product webpage

image

image 1 :

Flow Cytometry: MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 100uM pervanadate for 10 mins. (dark orange filled histogram), then stained with MBS629762 or control antibody (blue open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody. To facilitate intracellular staining, cells with fixed with paraformaldehyde and permeabilized with methanol.

image 2 :

Immunhistochemical staining of immersion fixed, frozen sections of mouse embryo (13 d.p.c) using MBS629762 at 15ug/ml (overnight incubation at 4 C. Tissue was stained using an anti-rabbit HRP-DAB staining kit and counterstained with hematoxylin (blue).

image 3 :

Western blot shows immunoprecipitate of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100uM pervanadate for 10 mins. PVDF membrane was probed with 0.5ug/ml MBS629762 , followed by HRP-conjugated anti-rabbit IgG secondary antibody. A specific band was detected for phospho HGF R/cMet (Y1234/Y1235) at ~145kD. Experiment was conducted under reducing conditions.

product information

catalog number :

MBS629762

products type :

Antibody

products full name :

c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

products short name :

[c-Met]

products name syn :

[Anti -c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)]

other names :

[c-Met; Hepatocyte growth factor receptor; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met]

other gene names :

[MET; MET; c-Met]

uniprot entry name :

MET_CANFA

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

specificity :

Detects human c-MET phosphorylated at Y1234/Y1235.

purity :

Affinity Purified. Purified by immunoaffinity chromatography

form :

Supplied as a lyophilized powder from a 0.2um filtered solution containing PBS, pH 7.4, 5% trehalose. Reconstitute with 250 ul sterile PBS.

concentration :

~0.2 mg/mL(after reconstitution)

storage stability :

Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Flow Cytometry, Immunohistochemistry,Western Blot. Other applications not tested.

app notes :

Flow Cytometry: 2.5ug/1x10e6 cells using MCF-7 human breast cancer cell line treated with pervanadate, fixed with paraformaldehyde and permealized with methanol. Immunohistochemistry: 5-15ug/ml using immersion fixed, frozen section of mouse embryo. Western Blot: 0.5ug/ml using pervanadate-treated MDA-MB-468 human breast cancer cell line . Optimal dilutions should be determined by each laboratory for each application.

image1 heading :

Flow Cytometry (FC/FACS)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen: Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites

products categories :

Antibodies; Abs to Growth Factors, Cytokines

products description :

Hepatocyte growth factor receptor (HGF R), also known as c-MET, is a receptor tyrosine kinase that consists of an extracellular chain and a longer chain. The chain contains the transmembrane helix and the cytoplasmic portion. Ligand-stimulated phosphorylation of tyrosine 1234 and tyrosine 1235 in HGF R's chain results in stimulation of intrinsic kinase activity.

ncbi gi num :

25815188

uniprot acc num :

Q75ZY9

ncbi mol weight :

154,637 Da

ncbi pathways :

Adherens Junction Pathway (84120); Adherens Junction Pathway (481); Axon Guidance Pathway (84115); Axon Guidance Pathway (476); Axon Guidance Pathway (364095); Bacterial Invasion Of Epithelial Cells Pathway (149829); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102295)

uniprot summary :

Function: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10. Subcellular location: Membrane; Single-pass type I membrane protein. Domain: The kinase domain is involved in SPSB1 binding. Post-translational modification: Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.

size1 :

0.05 mg

price1 :

670 USD

more info or order :

MyBioSource product webpage