antibody

14-3-3, phosphorylated (Ser185)

MBS628830

0.1 mg

560 USD

polyclonal

sheep

nonconjugated

human, mouse, rat, cow

Antibody

14-3-3, phosphorylated (Ser185)

14-3-3

Anti -14-3-3, phosphorylated (Ser185)

14-3-3 protein theta; 14-3-3 protein theta; 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

YWHAQ; YWHAQ; 1C5; HS1; 14-3-3

1433T_HUMAN

Polyclonal

IgG

Sheep

Bovine, Human, Mouse, Rabbit, Rat, Sheep

Recognizes sheep 1433 at ~30kD when phosphorylated at Ser185. Species Crossreactivity: human, bovine, mouse, rat and rabbit.

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid, 0.09% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

Suitable for use in Western Blot. Dilution: Western Blot: 1:100-1:300

Immunogen: Synthetic peptide, EILNSPEKAC, corresponding to 1433 beta and zeta phosphorylated at serine 185.

Antibodies; Abs to 14-3-3

5803227

NP_006817.1

NM_006826.3

P27348

27,764 Da

Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020!!Epstein-Barr Virus Infection Pathway 585562

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. Ref.12. Subunit structure: Homodimer. Interacts with CDK16 . By similarity. Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with GAB2. Interacts with the 'Ser-241' phosphorylated form of PDPK1. Ref.11 Ref.12 Ref.13 Ref.15 Ref.23. Subcellular location: Cytoplasm. Note: In neurons, axonally transported to the nerve terminals. Tissue specificity: Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. Ref.10. Post-translational modification: Ser-232 is probably phosphorylated by CK1. Ref.9. Sequence similarities: Belongs to the 14-3-3 family.

0.1 mg

560 USD