Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase) | MyBioSource MBS628517 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

catalog :

MBS628517

quantity :

0.1 mL

price :

635 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis using MBS628517.

image 2 :

Immunhistochemistry analysis of retina tissue using MBS628517. MBS628517 labels the two amacrine cells in this light-stimulated retina example

product information

catalog number :

MBS628517

products type :

Antibody

products full name :

Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

products short name :

[Tyrosine Hydroxylase]

products name syn :

[Anti -Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)]

other names :

[tyrosine hydroxylase; Tyrosine hydroxylase; tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase; tyrosine hydroxylase]

other gene names :

[TH; TYH; DYT14; DYT5b]

uniprot entry name :

O43816_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

specificity :

Recognizes the rat ~60kD tyrosine hydroxylase protein, phosphorylated at Ser40 and does not react with the non-phosphorylated form of the TH protein. Reactivity has been confirmed in rat PC-12 cell lysates stimulated with okadaic acid.

purity :

Purified by immunoaffinity chromatography

form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

storage stability :

Store at -20°C. (May be stored at 4°C for short-term only.). Aliquot to avoid repeated freezing and thawing. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluroescence (IF), Immunohistochemistry (IHC)

app notes :

Recommended Dilution:. Western Blot: 1:1000. Immunohistochemistry: 1:1000. Immunofluorescence: 1:1000. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Grade: Affinity Purified. Immunogen: Synthetic phospho-peptide derived from the rat tyrosine hydroxylase phosphorylated (Ser40).

products categories :

Antibodies; Abs to Enzymes

products description :

Tyrosine hydroxylase (TH, tyrosine 3-monooxygenase) is the eukaryotic enzyme that catalyzes the first, rate-limiting step in the biosynthesis of dopamine and other catecholamines from tyrosine.1 TH requires O2 and ferrous iron for the hydroxylation reaction it catalyzes. In mammals, TH is encoded by a single gene that is required for embryonic development and survival;2 full-length cDNA for this enzyme has been obtained for rat, mouse, bovine, Drosophila, macaque monkey, and human species.3 In all vertebrates, TH activity is closely regulated at many levels of expression, including gene transcription, RNA processing, stability of mRNA, feedback inhibition on enzyme, and protein kinase activation.2 The active tyrosine hydroxylase enzyme is a tetramer, with its active site located within its C-terminal region, where the physiological co-substrate binds. The N-terminus acts as a regulatory domain, and contains four serine residues, including Ser 19, Ser 31, and Ser40, that can be phosphorylated by protein kinase A (PKA), CaM kinase II (CaMKII), and protein kinase C (PKC).3 Ser40 is the major TH phosphorylation site of PKA and CaMKII in vertebrates.2,3 Phosporylation of TH at Ser40, and thus the synthesis of dopamine, has been observed to be influenced by many factors, including potassium ion concentration,4 activation of adenylyl cyclase,5 NMDA receptor activity,5 ERK1/2 activity,6,7 and the binding of 14-3-3 proteins.8

ncbi gi num :

986909

uniprot acc num :

O43816

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Alpha-synuclein Signaling Pathway (137913); Amine-derived Hormones Pathway (160983); Biogenic Amine Synthesis Pathway (198793); Biosynthesis Of Amine And Peptide Hormones Pathway (366244); Catecholamine Biosynthesis Pathway (160984); Dopamine Neurotransmitter Release Cycle Pathway (106523); Metabolic Pathways (132956); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Hormones Pathway (366243)

ncbi summary :

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

uniprot summary :

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: EC 1.14.16.2; Mitochondrial; Amino Acid Metabolism - tyrosine; Vesicle; Oxidoreductase; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus. Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity. Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; circadian sleep/wake cycle; response to ethanol; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; regulation of heart contraction; response to salt stress; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis. Disease: Segawa Syndrome, Autosomal Recessive

size1 :

0.1 mL

price1 :

635 USD

more info or order :

MyBioSource product webpage