antibody

IKK alpha, beta, phosphorylated (Ser180/181) (IkappaB Kinase alpha, beta, IKKa,b)

MBS627082

0.05 mg

390 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

IKK alpha, beta, phosphorylated (Ser180/181) (IkappaB Kinase alpha, beta, IKKa,b)

IKK alpha, beta

Anti -IKK alpha, beta, phosphorylated (Ser180/181) (IkappaB Kinase alpha, beta, IKKa,b)

inhibitor of nuclear factor kappa-B kinase subunit beta isoform 1; Inhibitor of nuclear factor kappa-B kinase subunit beta; inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta

IKBKB; IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta; IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB

IKKB_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

Recognizes human IKK alpha, beta only when phosphorylated at serine 180/181. Species Crossreactivity: mouse, rat.

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:4000. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100

Immunogen: Synthetic phosphopeptide corresponding to human IKKa/b around the phosphorylation site of serine 180/181 (C-T-pS-F-V).

Antibodies; Abs to Protein Kinases

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkappaB. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunit of the kinase. IKKgamma serves as its regulatory subunit. Activation of IKK depends on phosphorylation, and the serines 177 and 181 in the activation loop of IKKbeta (176 and 180 in IKKalpha) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation.

41352712

NP_001547.1

NM_001556.2

O14920

86,564 Da

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (106400); Activation Of NF-kappaB In B Cells Pathway (576251); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Apoptosis Pathway (198797); Apoptosis Pathway (83060)

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer. Protein type: EC 2.7.11.10; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family. Chromosomal Location of Human Ortholog: 8p11.2. Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft. Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity. Biological Process: I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to virus; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; negative regulation of apoptosis. Disease: Immunodeficiency 15

0.05 mg

390 USD