antibody

Phospholipase C, gamma 2, phosphorylated (Tyr1217)

MBS626427

0.1 mg

520 USD

polyclonal

rabbit

nonconjugated

Antibody

Phospholipase C, gamma 2, phosphorylated (Tyr1217)

[Phospholipase C, gamma 2]

[Anti -Phospholipase C, gamma 2, phosphorylated (Tyr1217)]

[phospholipase C, gamma 2 (phosphatidylinositol-specific), isoform CRA_a; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; PLC-IV; PLC-gamma-2; phospholipase C-IV; phospholipase C gamma 2; phospholipase C-gamma-2; phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylyinositol-specific); phospholipase C, gamma 2 (phosphatidylinositol-specific); Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2]

[PLCG2; PLCG2]

PLCG2_HUMAN

Polyclonal

IgG

Rabbit

Human

Recognizes endogenous levels of human PLC-gamma 2 only when phosphorylated at tyrosine 1217.

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

1mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry (Paraffin sections): 1:50-1:100

Immunohistochemistry (IHC)

Immunogen: Synthesized phosphopeptide derived from human PLC-gamma2 around the phosphorylation site of tyrosine 1217 (F-L-pY-D-T).

Antibodies; Abs to Enzymes, Phospholipase

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate. PLCgII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgII. Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk.

119615931

P16885

147,870 Da

B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class I PI3K Signaling Events Pathway (138022); D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway (138940); D-myo-inositol-5-phosphate Metabolism Pathway (138601); EGFR1 Signaling Pathway (198782)

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1; MIM 172420) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. The C-gamma-1 and C-gamma-2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.[supplied by OMIM]

PLCG2: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. It is a crucial enzyme in transmembrane signaling. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors. Protein type: Carbohydrate Metabolism - inositol phosphate; Phospholipase; EC 3.1.4.11; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16q24.1. Cellular Component: plasma membrane; cytosol. Molecular Function: signal transducer activity; protein binding; phospholipase C activity; phosphoinositide phospholipase C activity. Biological Process: platelet activation; inositol phosphate metabolic process; Wnt receptor signaling pathway; phospholipid catabolic process; calcium-mediated signaling; response to lipopolysaccharide; T cell receptor signaling pathway; activation of store-operated calcium channel activity; B cell receptor signaling pathway; inositol trisphosphate biosynthetic process; regulation of gene expression; release of sequestered calcium ion into cytosol; B cell differentiation; phosphatidylinositol biosynthetic process; negative regulation of programmed cell death; innate immune response; follicular B cell differentiation; blood coagulation. Disease: Autoinflammation, Antibody Deficiency, And Immune Dysregulation, Plcg2-associated; Familial Cold Autoinflammatory Syndrome 3

0.1 mg

520 USD