antibody

c-Met, Phosphorylated (Tyr1230, Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

MBS626131

0.1 mL

540 USD

polyclonal

rabbit

nonconjugated

human, mouse, dog

Antibody

c-Met, Phosphorylated (Tyr1230, Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

c-Met

Anti -c-Met, Phosphorylated (Tyr1230, Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

c-Met; Hepatocyte growth factor receptor; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

MET; MET; c-Met

MET_CANFA

Polyclonal

IgG

Rabbit

Human, Mouse

Recognizes phosphorylated c-Met (Tyr1230/Tyr1234/Tyr1235). Species Cross-reactivity

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

Suitable for use in Western Blot. Dilution: Western Blot: 1:2000 detects phospho-Met (Tyr1230/Tyr1234/Tyr1235) in A431 cells treated with EGF.

Immunogen: Synthetic peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-c-Met (Tyr1230/Tyr1234/Tyr1235).

Antibodies; Abs to Growth Factors, Cytokines

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c-Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c-Met contains three tyrosines (Tyr-xx-

25815188

Q75ZY9

154,637 Da

Adherens Junction Pathway (84120); Adherens Junction Pathway (481); Axon Guidance Pathway (84115); Axon Guidance Pathway (476); Axon Guidance Pathway (364095); Bacterial Invasion Of Epithelial Cells Pathway (149829); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102295)

Function: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10. Subcellular location: Membrane; Single-pass type I membrane protein. Domain: The kinase domain is involved in SPSB1 binding. Post-translational modification: Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.

0.1 mL

540 USD