catalog number :
MBS625591
products full name :
ARF, p14 (p14ARF)
products short name :
[ARF, p14]
products name syn :
[Anti -ARF, p14 (p14ARF)]
reactivity :
This antibody reacts with human p14ARF. Other species untested.
specificity :
This antibody is specific for p14ARF.
purity :
Affinity Purified. Purified by immunoaffinity chromatography.
form :
Supplied as a liquid in Tri-Citrate/Phosphate, 0.09% sodium azide.
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
Western Blot (WB), Immunofluorescence (IF)
app notes :
Suitable for use in Immunofluorescence and Western Blot.
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Immunogen: A synthetic peptide mapping to a fragment of residues within amino acids 75-125 of human p14ARF.
other info2 :
Positive Control: BT549 or HeLa whole cell extracts
products categories :
Antibodies; Abs to Cancer Markers
products description :
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.