IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1) | MyBioSource MBS624304 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)

catalog :

MBS624304

quantity :

0.1 mL

price :

635 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot using MBS624304.

image 2 :

Immunohistochemistry analysis of Phospho-IRS1 [pY612] showing staining in the cytoplasm and nucleus of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with MBS624304 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4 C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

image 3 :

Flow cytometry analysis of IRS1 [pY612] was done on MCF7 cells treated with Insulin (100nM, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at RT. Cells were labeled with MBS624304 (red histogram) or with rabbit isotype control (pink histogram) at 3-5ug/million cells in 2.5% BSA. After incubation at RT for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at RT. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

product information

catalog number :

MBS624304

products type :

Antibody

products full name :

IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)

products short name :

[IRS-1]

products name syn :

[Anti -IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)]

other names :

[insulin receptor substrate 1; Insulin receptor substrate 1; insulin receptor substrate 1; IRS-1; OTTHUMP00000164234; insulin receptor substrate 1]

other gene names :

[IRS1; IRS1; HIRS-1]

uniprot entry name :

IRS1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse

specificity :

Recognizes human IRS-1 when phosphorylated at Tyr612

purity :

Affinity Purified. Purified by immunoaffinity chromatography.

form :

Supplied as a liquid in PBS, pH 7.3, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

concentration :

0.25 mg/mL

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Flow Cytometry (FC), Western Blot (WB), Immunohistochemistry (IHC). Other applications not tested.

app notes :

Recommended Dilution:. Immunohistochemistry (Paraffin): 1:10-1:100 . Flow Cytometry: 3-5ug/million cells. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Immunogen: Synthetic phosphopeptide derived from a region of human IRS-1 that contains tyrosine 612. Species sequence homology: rat, chicken. Positive Control: CHO-T cells transiently transfected with human IRS-1

products categories :

Antibodies; Abs to Protein Kinase Substrates

products description :

Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm"e;s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.

ncbi gi num :

5031805

ncbi acc num :

NP_005535.1

ncbi gb acc num :

NM_005544.2

uniprot acc num :

P35568

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Downstream Signaling Of Activated FGFR Pathway (160957); EPO Receptor Signaling Pathway (198882); IGF1 Pathway (137992); IL-2 Signaling Pathway (198885)

ncbi summary :

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

uniprot summary :

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 2q36. Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol. Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding. Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

0.1 mL

price1 :

635 USD

more info or order :

MyBioSource product webpage