antibody

Bacillus anthracis PA, Protective Antigen (Anthrax)

MBS623626

0.1 mg

575 USD

polyclonal

goat

nonconjugated

Antibody

Bacillus anthracis PA, Protective Antigen (Anthrax)

Bacillus anthracis PA, Protective Antigen

Anti -Bacillus anthracis PA, Protective Antigen (Anthrax)

Bacillus anthracis protective antigen

Polyclonal

IgG

Goat

Recognizes bothPA63 and PA83 of B. anthracis Lethal Factor. No cross-reactivity with Lethal Factor (LF) of Bacillus anthracis, Y. Pestis, F. Tularensis and Toxoplasma gondi.

Grade: Purified. Purified

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

1mg/ml

May be stored at 4 degree C for short term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB). Other applications have not tested.

Suitable for use in ELISA and Western Blot. Dilution: Western Blot (ECL): 1-10ug/ml. ELISA: 0.1-1ug/ml. Optimal dilutions to be determined by the researcher.

Immunogen: Recombinant corresponding to purified Bacillus anthracis Protective Antigen (PA83).

Antibodies; Abs to Infectious Disease Anthrax

After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker-8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kD fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells.

156255913

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

0.1 mg

575 USD