Nitrotyrosine (nTyr) | MyBioSource MBS622995 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Nitrotyrosine (nTyr)

catalog :

MBS622995

quantity :

0.1 mL

price :

570 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

cow

more info or order :

MyBioSource product webpage

image

image 1 :

Nitrotyrosine antibody specificity. Competition for MBS622995 binding to a NT containing 13mer peptide coated on a plate. ELISA competitors are free nitrotyrosine (diamond), NT containing 33mer peptide (circle), chlorotyrosine (square), and tyrosine (triangle). Competition for Ab binding was most effective with free NT followed closely by the NT containing 33mer peptide. This shows that MBS622995 will recognize free NT as well as NT containing proteins in biological fluids. Significant competition by tyrosine and modified tyrosine occurred at greater than two orders of magnitude higher concentrations of each, indicating very good NT Ab specificity.

image 2 :

Western Blot using nitrotyrosine immunoblot positive control on nitrocellulose with MBS622995 at 1:1000 dilution. Lane 1, 2.44ug; Lane 2, 9.75ng positive control (nitrated carbonic anhydrase).

product information

catalog number :

MBS622995

products type :

Antibody

products full name :

Nitrotyrosine (nTyr)

products short name :

[Nitrotyrosine]

products name syn :

[Anti -Nitrotyrosine (nTyr)]

other names :

[nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible<; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2]

other gene names :

[NOS2; NOS2; iNOS; NOS2A; Inducible NOS; iNOS; NOSII]

uniprot entry name :

NOS2_BOVIN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

specificity :

Recognizes free Nitrotyrosine and nitrotyrosine-containing proteins.

purity :

Purififed by Ig salt fraction.

form :

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

concentration :

40 mg/ml (Total protein).

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in ELISA (EIA), Western Blot (WB) and Immunohistochemistry (IHC). Other applications not tested.

app notes :

Western Blot 1:1000. Immunohistochemistry: 1:100-1:10,000. Optimal dilutions to be determined by the researcher.

image1 heading :

ELISA (EIA)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: Nitrated KLH

other info2 :

Positive Control: MBS657779: Nitrotyrosine, Immunoblot Positive Control (nTyr).

products categories :

Antibodies; Abs to Nitrotyrosine

products description :

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

ncbi gi num :

116003827

ncbi acc num :

NP_001070267.1

ncbi gb acc num :

NM_001076799.1

uniprot acc num :

Q27995

ncbi pathways :

Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (84202); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Disease Pathway (827627); HIF-1 Signaling Pathway (695207)

uniprot summary :

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . By similarity. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . By similarity.Binds 1 FAD . By similarity.Binds 1 FMN . By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme . By similarity. Enzyme regulation: Regulated by calcium/calmodulin . By similarity. Subunit structure: Homodimer. Binds SLC9A3R1 . By similarity. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

size1 :

0.1 mL

price1 :

570 USD

more info or order :

MyBioSource product webpage