Cytokeratin 8,18 | MyBioSource MBS621174 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Cytokeratin 8,18

catalog :

MBS621174

quantity :

0.1 mL

price :

695 USD

clonality :

polyclonal

host :

guinea pigs

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS621174

products type :

Antibody

products full name :

Cytokeratin 8,18

products short name :

[Cytokeratin 8,18]

products name syn :

[Anti -Cytokeratin 8,18]

other names :

[cytokeratin 8 (279 AA); Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8]

other gene names :

[KRT8; KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; CYK8; CK-8; K8]

uniprot entry name :

K2C8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Guinea pig

reactivity :

Human, rat, mouse, Syrian hamster, Xenopus laevis, chicken.

specificity :

Recognizes bovine Cytokeratin 8,18 of simple type epithelium at ~52.5kD and 45.5kD respectively. Detected all adeno-Ca tested, undifferentiated CA, hepatocellular CA and cervix Ca tumors.

purity :

Serum

form :

Supplied as a liquid, 0.09% sodium azide.

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Other applications have not been tested.

app notes :

Western Blot: 1:2000. Immunohistochemistry (Frozen): 1:100. Immunohistochemistry (Paraffin): 1:50. Microwave treatment recommended. Optimal dilutions to be determined by the researcher.

other info1 :

Immunogen: Cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18.

products categories :

Antibodies; Abs to Cytokeratin

products description :

Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.

ncbi gi num :

30313

ncbi acc num :

CAA31376.1

uniprot acc num :

P05787

ncbi pathways :

EGFR1 Signaling Pathway (198782); Signaling Mediated By P38-alpha And P38-beta Pathway (138012)

ncbi summary :

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma. Molecular Function: protein binding; protein complex binding; structural molecule activity. Biological Process: viral reproduction; tumor necrosis factor-mediated signaling pathway; response to other organism; sarcomere organization; response to hydrostatic pressure. Disease: Cirrhosis, Familial

size1 :

0.1 mL

price1 :

695 USD

more info or order :

MyBioSource product webpage