ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5) | MyBioSource MBS621147 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5)

catalog :

MBS621147

quantity :

0.1 mg

price :

610 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Positive and background staining patterns on formalin-fixed, paraffin-embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Stained using MBS621147 diluted to 1:300 and detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm.

product information

catalog number :

MBS621147

products type :

Antibody

products full name :

ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5)

products short name :

[ASPM]

products name syn :

[Anti -ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, FLJ10517, FLJ10549, MCPH5)]

other names :

[ASPM protein; Abnormal spindle-like microcephaly associated splice variant 2; abnormal spindle-like microcephaly-associated protein; OTTHUMP00000034411; OTTHUMP00000034412; asp (abnormal spindle) homolog, microcephaly associated (Drosophila)]

other gene names :

[ASPM; ASPM; ASP; MCPH5; Calmbp1; FLJ10517; FLJ10549; FLJ43117; DKFZp686N06184]

uniprot entry name :

Q4G1H0_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Rat

specificity :

Recognizes human ASPM. Species Crossreactivity: rat.

purity :

Affinity Purified. Purified by affinity chromatography.

form :

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

concentration :

1mg/ml

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

ELISA (EL/EIA), Immunohistochemistry (IHC)

app notes :

Suitable for use in ELISA and Immunohistochemistry. Dilution: ELISA: Tested in direct ELISA against the ASPM immunogen. Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Linear peptide corresponding to human ASPM conjugated to KLH. (NP_060606). Species Sequence Homology: Chimpanzee and primate. Epitope: internal

other info2 :

Positive Control: Human ductal carcinoma tissue

products categories :

Antibodies; Abs to Proteins

products description :

ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.

ncbi gi num :

34193569

uniprot acc num :

Q4G1H0

ncbi mol weight :

218, 95, 75kD Observed UniProtKB/ Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800 kD and Isoform 2 at 217.794 kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol.14 No. 15 2155-2165.

ncbi summary :

This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.

uniprot summary :

ASPM: Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis. Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5); also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation. Chromosomal Location of Human Ortholog: 1q31. Cellular Component: microtubule; cytoplasm; midbody; nucleus. Molecular Function: calmodulin binding. Biological Process: oogenesis; developmental growth; maintenance of centrosome localization; mitosis; positive regulation of neuroblast proliferation; negative regulation of neuron differentiation; negative regulation of asymmetric cell division; male gonad development; neuron migration; spermatogenesis; cerebral cortex development; forebrain neuroblast division. Disease: Microcephaly 5, Primary, Autosomal Recessive

size1 :

0.1 mg

price1 :

610 USD

more info or order :

MyBioSource product webpage