ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4) | MyBioSource MBS620136 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4)

catalog :

MBS620136

quantity :

0.1 mg

price :

765 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS620136

products type :

Antibody

products full name :

ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4)

products short name :

[ADAMTS4]

products name syn :

[Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4)]

other names :

[A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; OTTHUMP00000032249; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADMP-1; Aggrecanase-1]

other gene names :

[ADAMTS4; ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688; KIAA0688]

uniprot entry name :

ATS4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Goat

reactivity :

Human

specificity :

Recognizes human ADAMTS4. Shows ~10% crossreactivity with recombinant human ADAMTS5 and <1% crossreactivity with recombinant human ADAMTS13, ADAMTS L1.2 and ADAMTS1 in Western Blot and ELISA.

purity :

Affinity Purified. Purified by immunoaffinity chromatography

form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

storage stability :

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 0.1ug/ml. Immunoprecipitation: 25ug/ml immunoprecipitates ADAMTS4 from conditioned cell culture medium spiked with recombinant human ADAMTS4.

other info1 :

Immunogen: Recombinant corresponding to Phe213-Cys685 from human ADAMTS4 expressedin CHO cells (O75173).

products categories :

Antibodies; Abs to Matrix Metalloproteinases

products description :

ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified recombinant human ADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5mM 1,10-phenanthroline and recombinant human TIMP-3.

ncbi gi num :

157427675

ncbi acc num :

NP_005090.3

ncbi gb acc num :

NM_005099.4

uniprot acc num :

O75173

ncbi mol weight :

90,197 Da

ncbi pathways :

Endochondral Ossification Pathway (198812)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq]

uniprot summary :

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the 392-Glu- -Ala-393 site. Protein type: Secreted, signal peptide; Protease; EC 3.4.24.82; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 1q21-q23. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: peptidase activity; protein binding; protease binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development

size1 :

0.1 mg

price1 :

765 USD

more info or order :

MyBioSource product webpage