PP2A-B' (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B') | MyBioSource MBS619224 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PP2A-B' (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B')

catalog :

MBS619224

quantity :

0.1 mg

price :

620 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow, fruit fly

application :

western blot, other

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot Analysis using MBS619224: Representative lot data Lane 1: PC12 lysate Lane 2: A431 lysate Lane 3: HepG2 lysate Lane 4: HeLa lysate Lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS619224. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Arrow indicates protein MBS619224 (~56kD).

product information

catalog number :

MBS619224

products type :

Antibody

products full name :

products short name :

[PP2A-B']

products name syn :

[Anti -PP2A-B' (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B')]

other names :

[PP2A-B', isoform I; PP2A-B', isoform K; CG7913 gene product from transcript CG7913-RK; PP2A-B', isoform K; CG7913-PK; PP2A-B'-PK; PP2A B' regulatory subunit; well rounded; regulatory B' subunit of PP2A; protein phosphatase 2A; protein phosphatase 2A-B'; protein phosphatase 2A regulatory B subunit; well-rounded; PP2A B' subunit; PP2A-B', isoform J; CG7913-PJ; PP2A-B'-PJ; PP2A-B', isoform E; CG7913-PE; PP2A-B'-PE; PP2A-B', isoform D; CG7913-PD; PP2A-B'-PD; PP2A-B', isoform I; CG7913-PI; PP2A-B'-PI; PP2A-B', isoform C; CG7913-PC; PP2A-B'-PC; PP2A-B', isoform B; CG7913-PB; PP2A-B'-PB; PP2A-B', isoform A; CG7913-PA; PP2A-B'-PA]

other gene names :

[PP2A-B'; PP2A-B'; anon-WO0118547.420; B'; B'/PR61; B56-1; BcDNA:GM05554; CG 7913; CG7901; CG7913; dB56-1; DmelCG7913; dPP2A; i234; PP2A; PP2A B'; PP2A-B; wrd]

uniprot entry name :

Q5BIJ2_DROME

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Human, Mouse, Rat

specificity :

Recognizes human PP2A-B' subunits. Species Crossreactivity: bovine, mouse, rat.

purity :

Purified

form :

Supplied as a liquid in PBS, 0.08% sodium azide.

concentration :

1 mg/mL

storage stability :

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months .For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

Suitable for use in Western Blot. Other applications not tested.

app notes :

Western Blot: 1:500-1:1500. Optimal dilutions to be determined by the researcher.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide corresponding to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH, conjugated to KLH. Epitope: Internal.

other info2 :

Positive Control: HeLa Cell Lysate

products categories :

Antibodies; Abs to Enzymes, Phosphatase

products description :

PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma. The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.

ncbi gi num :

221379828

ncbi acc num :

NM_006243

ncbi gb acc num :

NM_001144600.1

uniprot acc num :

Q5BIJ2

ncbi pathways :

Activated TLR4 Signalling Pathway (365443); Adaptive Immunity Signaling Pathway (365412); Beta-catenin Phosphorylation Cascade Pathway (365705); CTLA4 Inhibitory Signaling Pathway (365419); Costimulation By The CD28 Family Pathway (365415); Degradation Of Beta-catenin By The Destruction Complex Pathway (365704); ERK/MAPK Targets Pathway (365431); ERKs Are Inactivated Pathway (365432); Glucose Metabolism Pathway (365586); Glycogen Metabolism Pathway (198276)

size1 :

0.1 mg

price1 :

620 USD

more info or order :

MyBioSource product webpage