Mast Cell Chymase (CMA1) | MyBioSource MBS618805 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mast Cell Chymase (CMA1)

catalog :

MBS618805

quantity :

0.1 mg

price :

780 USD

clonality :

polyclonal

host :

sheep

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot of human Chymase/CMA1using MBS618805

product information

catalog number :

MBS618805

products type :

Antibody

products full name :

Mast Cell Chymase (CMA1)

products short name :

[Mast Cell Chymase]

products name syn :

[Anti -Mast Cell Chymase (CMA1)]

other names :

[mast cell chymase; Chymase; chymase; alpha-chymase; chymase, heart; OTTHUMP00000178751; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I]

other gene names :

[CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM]

uniprot entry name :

CMA1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Sheep

reactivity :

Human

specificity :

Recognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rhGranzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7.

purity :

Affinity Purified. Purified by immunoaffinity chromatography.

form :

Supplied as a lyophilized powder from PBS, ph7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

concentration :

~0.2mg/ml (after reconstitution)

storage stability :

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Suitable for use in Western Blot, Immunoprecipitation and ELISA. Dilution: Western Blot: 0.1-0.2ug/ml. Detection limit is ~5ng/lane under non-reducing or reducing conditions. Immunoprecipitation: Immunoprecipitate rhChymase. from conditioned media of transfected NS0 cells. ELISA: 0.5-1ug/ml. Detection limit is ~1.5ng/well.

image1 heading :

Western Blot (WB)

other info1 :

Grade: Affinity Purified. Immunogen: Recombinant protein corresponding to Gly20-Asn247 from human Chymase expressed in NS0 cells.

products categories :

Antibodies; Abs to Cell Markers

products description :

Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa22-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa49-247).

ncbi gi num :

180542

uniprot acc num :

P23946

ncbi pathways :

ACE Inhibitor Pathway (198763); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq]

uniprot summary :

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Secreted, signal peptide; EC 3.4.21.39; Secreted; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding. Biological Process: interleukin-1 beta biosynthetic process; extracellular matrix disassembly; positive regulation of angiogenesis; extracellular matrix organization and biogenesis; cellular protein metabolic process; regulation of inflammatory response; midbrain development; protein processing; angiotensin maturation; proteolysis

size1 :

0.1 mg

price1 :

780 USD

more info or order :

MyBioSource product webpage