antibody

Glutamate Decarboxylase 65,67 (GAD 65,67)

MBS618215

0.1 mL

645 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Glutamate Decarboxylase 65,67 (GAD 65,67)

Glutamate Decarboxylase 65,67

Anti -Glutamate Decarboxylase 65,67 (GAD 65,67)

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

GAD2; GAD2; GAD65; GAD65; GAD-65

DCE2_HUMAN

Polyclonal

IgG

Rabbit

Feline, Human, Mouse

Recognizes both molecular forms of rat glutamic acid decarboxylase (GAD65 and GAD67), the enzyme which converts glutamic acid to gama-aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.

Purified. Purified by ammonium sulfate precipitation.

Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunofluorescence. Dilution: Immunohistochemistry (Frozen): 1:200-1:5000. Immunohistochemistry: Paraffin sections. Western Blot: 1:1000-1:10,000. A doublet at ~65/67kD is expected. Optimal working dilutions to be determined by researcher. Recommended Positive Control Tissues: Brain

Immunogen: Synthetic peptide corresponding to DFLIEEIERLGQDL from the C-terminus of rat GAD.

Antibodies; Abs to Enzymes, Decarboxylase

Glutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585aa) encoded on human chromosome10. GAD67 is cytoplasmic (594aa) and is encoded on chromosome 2. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). Changes in GAD65/GAD67 distributions have also been correlated with certain disease states such as IDDM and SMS.

4503875

NP_000809.1

NM_000818.2

Q05329

65,411 Da

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine; Lyase; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cell junction; cytosol. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

0.1 mL

645 USD