antibody

Myelin Basic Protein, Degraded (MBP)

MBS618031

0.05 mL

585 USD

polyclonal

rabbit

nonconjugated

Antibody

Myelin Basic Protein, Degraded (MBP)

Myelin Basic Protein, Degraded

Anti -Myelin Basic Protein, Degraded (MBP)

myelin basic protein isoform 4; Myelin basic protein S; Golli-Mbp; myelin basic protein; MBP S; myelin basic protein S; myelin basic protein Golli-mbp; Golli-Mbp; myelin basic protein; myelin basic protein S; myelin basic protein

Mbp; Mbp; Mbps

MBP_RAT

Polyclonal

IgG

Rabbit

Guinea Pig, Human, Rat

Recognizes Myelin Basic Protein in demyelinated nerve tissues. High specificity for lesioned rat spinal cord. Species Crossreactivity: human and rat.

Serum. Serum

Supplied as a lyophilized powder. Reconstitute with 50ul of sterile ddH2O. Preservative free.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry: 1:2000. Immunostains myelin basic protein of abnormal appearing oligodendrocytic processes and cell bodies in demyelinating areas. Recognizes only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerve. It also stains discrete white matter in brain of multiple system atrophy. Optimal dilution determined by the researcher.

Type: Pab. Immunogen: Synthetic peptide corresponding to aa69-86 of the guinea pig Myelin Basic Protein, Degraded.

Antibodies; Abs to Myelin

Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles (3). MBP serves as a marker for oligodendrocytes and Schwann cells (3). MBP is also a marker for malignant schwannomas (4) and appears to play a significant role in the etiology of multiple sclerosis (5-7) and experimental autoimmune encephalomyelitis (8).

70166270

NP_001020465.1

NM_001025294.1

P02688

21,502 Da

MAPK Cascade Pathway (198535)

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq]

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Cellular Component: internode region of axon; compact myelin; cell projection; cell soma; plasma membrane; myelin sheath. Molecular Function: protein binding; protease binding; structural constituent of myelin sheath. Biological Process: response to mercury ion; myelination; substantia nigra development; response to toxin; negative regulation of axonogenesis; response to progesterone stimulus; aging

0.05 mL

585 USD