antibody

Glucocorticoid Receptor beta (GRb)

MBS617111

0.05 mL

755 USD

polyclonal

rabbit

nonconjugated

Antibody

Glucocorticoid Receptor beta (GRb)

[Glucocorticoid Receptor beta]

[Anti -Glucocorticoid Receptor beta (GRb)]

[glucocorticoid receptor isoform beta; Glucocorticoid receptor; glucocorticoid receptor; OTTHUMP00000160084; OTTHUMP00000160085; OTTHUMP00000222851; OTTHUMP00000222854; OTTHUMP00000222855; OTTHUMP00000222858; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1]

[NR3C1; NR3C1; GR; GCR; GRL; GCCR; GRL]

GCR_HUMAN

Polyclonal

IgG

Rabbit

Recognizes human GRb at ~90kD.

Serum

Supplied as a liquid in PBS, 0.05% sodium azide.

Not determined

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Suitable for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Other applications not tested.

Western Blot: 1:500. Immunocytochemistry: 1:1500. Optimal dilutions to be determined by the researcher.

Western Blot (WB)

Immunogen: Synthetic peptide corresponding to aa 728-742 [NVMWLKPESTSHTLI] of human GRb.

Antibodies; Abs to Receptors

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94kD GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90kD GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.

66528642

NP_001018661.1

NM_001020825.1

P04150

Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); IL-2 Signaling Pathway (198885); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq]

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. Protein type: Mitochondrial; Nuclear receptor; Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus. Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter. Disease: Glucocorticoid Resistance, Generalized

0.05 mL

755 USD