antibody

MYPT1, phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1)

MBS616540

0.2 mg

595 USD

polyclonal

rabbit

nonconjugated

human, mouse, chicken

Antibody

MYPT1, phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1)

MYPT1

Anti -MYPT1, phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1)

myosin phosphatase target subunit 1; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1 regulatory subunit 12A; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase myosin-binding subunit; protein phosphatase 1, regulatory (inhibitor) subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit

PPP1R12A; PPP1R12A; MBS; MYPT1; MGC133042; MBS; MYPT1

MYPT1_HUMAN

Polyclonal

IgG

Rabbit

Chicken, Human, Mouse, Rat

Recognizes recombinant truncated MYPT1 phosphorylated on Thr853, MW ~80kD. Species Crossreactivity: human (Thr853, O014974), chicken (Thr850, Q90623), mouse (Thr852, Q9DBR7) and rat (Thr855, Q10728).

Affinity Purified. Purified by Protein A affinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

1 mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

Suitable for use in Western Blot. Dilution: Western Blot: 0.5-2ug/ml. Detects MYPT1 in C2C12 lysates treated with pervanadate.

Immunogen: Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes (Q90623). Phosphorylation site corresponds to Thr853 in human MYPT1.

Antibodies; Abs to Myosin

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown (6). To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.

13365493

O14974

115,281 Da

Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Long-term Potentiation Pathway (83085); Long-term Potentiation Pathway (496); PLK1 Signaling Events Pathway (138007); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); RhoA Signaling Pathway (169356)

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit. Chromosomal Location of Human Ortholog: 12q15-q21. Cellular Component: nucleoplasm; kinetochore; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton. Molecular Function: enzyme inhibitor activity; signal transducer activity; protein binding; phosphatase regulator activity; protein kinase binding. Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; signal transduction; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation

0.2 mg

595 USD