Carbonic Anhydrase IX (CA9) | MyBioSource MBS616120 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Carbonic Anhydrase IX (CA9)

catalog :

MBS616120

quantity :

0.1 mL

price :

625 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Detection of carbonic anhydrase IX in transfected HEK cell lysate using MBS616120. Rabbit IgG was used as a negative control.

image 2 :

Immunohistochemistry analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer usingMBS616120 at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin.

image 3 :

Immunohistochemistry of frozen human colon carcinoma, xenografted in mice using MBS616120

product information

catalog number :

MBS616120

products type :

Antibody

products full name :

Carbonic Anhydrase IX (CA9)

products short name :

[Carbonic Anhydrase IX]

products name syn :

[Anti -Carbonic Anhydrase IX (CA9)]

other names :

[carbonic anhydrase IX; Carbonic anhydrase 9; carbonic anhydrase 9; pMW1; CA-IX; P54/58N; OTTHUMP00000022773; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250; carbonic anhydrase IX; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1]

other gene names :

[CA9; CA9; MN; CAIX; G250; MN]

uniprot entry name :

CAH9_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Canine, mouse and rat

specificity :

Recognizes human carbonic anhydrase IX.

purity :

Affinity Purified. Purified by immunoaffinity chromatography.

form :

Supplied as a liquid in PBS, pH 7.5, 0.025% sodium azide

concentration :

1.0 mg/ml

storage stability :

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

app notes :

Immunofluorescence (IC): 1:1000 . Western Blot: 1:2000. Flow Cytometry: 1:1000. Immunohistochemistry (Frozen, paraffin): 1:1000. Optimal dilutions to be determined by the researcher

image1 heading :

Testing Data

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence of HEK 293 cells using MBS616120. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.

other info1 :

Immunogen: Synthetic peptide corresponding to a C-terminal sequence of the human carbonic anhydrase IX. Cellular Localization: Cell Membranes

other info2 :

Positive Control: Renal carcinoma

products categories :

Antibodies; Abs to Enzymes

products description :

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

ncbi gi num :

119578763

uniprot acc num :

Q16790

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416)

ncbi summary :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

uniprot summary :

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: Membrane protein, integral; Nucleolus; Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: basolateral plasma membrane; microvillus membrane; nucleolus; integral to membrane; plasma membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to testosterone stimulus

size1 :

0.1 mL

price1 :

625 USD

more info or order :

MyBioSource product webpage