antibody

Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135)

MBS616026

0.2 mg

630 USD

polyclonal

rabbit

nonconjugated

human, mouse, dog

western blot, immunocytochemistry, immunoprecipitation

Antibody

Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135)

[Flt3, Cytoplasmic]

[Anti -Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135)]

[fms-related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand; Flt3 ligand]

[FLT3LG]

Polyclonal

IgG

Rabbit

Human, Mouse

Recognizes the processed (130kD) and the mature forms (155kD) of mouse Flt3. Species Crossreactivity: human

Purified by Protein A affinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

1 mg/ml

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Western Blot: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F4600-08 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitated Flt3 from 500ug of mouse Bafl-3 RIPA lysate. Immunocytochemistry: 10ug/ml showed positive immunostaining for Flt3 in mouse Bafl-3 cells fixed with methanol and permeabilized with 0.25% Triton X-100 for 5 mins.

Western Blot (WB)

Immunogen: His-tag fusion protein corresponding to aa786-1000 of mouse Flt3. Grade: Affinity Purified

Antibodies; Abs to Tyrosine Kinases

Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in vitro in cooperation with other cytokines. Flt3 is also expressed in the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells.

50979198

NP_001003350.1

NM_001003350.1

Cytokine-cytokine Receptor Interaction Pathway (84101); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (84128); Hematopoietic Cell Lineage Pathway (489); Pathways In Cancer (84151)

0.2 mg

630 USD