MyBioSource

antibody

ACSL5 (A-CoA Synthetase Long-chain Family Member 5, HGNC:16526, ACS2, ACS5, FACL5, Fatty-acid-Coenzyme A Ligase, Long-chain 5, Long-chain Acyl-CoA Synthetase 5)

MBS615984

0.1 mg

620 USD

polyclonal

goat

human

enzyme immunoassay, ELISA, western blot

MBS615984

Antibody

ACSL5 (A-CoA Synthetase Long-chain Family Member 5, HGNC:16526, ACS2, ACS5, FACL5, Fatty-acid-Coenzyme A Ligase, Long-chain 5, Long-chain Acyl-CoA Synthetase 5)

ACSL5

Anti -ACSL5 (A-CoA Synthetase Long-chain Family Member 5, HGNC:16526, ACS2, ACS5, FACL5, Fatty-acid-Coenzyme A Ligase, Long-chain 5, Long-chain Acyl-CoA Synthetase 5)

acyl-CoA synthetase long-chain family member 5; Long-chain-fatty-acid--CoA ligase 5; long-chain-fatty-acid--CoA ligase 5; LACS 5; OTTHUMP00000020489; OTTHUMP00000020490; fatty acid coenzyme A ligase 5; long-chain acyl-CoA synthetase 5; FACL5 for fatty acid coenzyme A ligase 5; long-chain fatty acid coenzyme A ligase 5; fatty-acid-Coenzyme A ligase, long-chain 5; acyl-CoA synthetase long-chain family member 5; Long-chain acyl-CoA synthetase 5

ACSL5; ACSL5; ACS2; ACS5; FACL5; ACS5; FACL5

ACSL5_HUMAN

Polyclonal

Host: Goat; Source: Human

Human

Species Crossreactivity: Human. Other species not tested.

Affinity Purified. Purified by immunoaffinity chromatography.

Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA (peptide) and Western Blot. Dilution: ELISA (peptide): 1:16,000. Western Blot: 1-3ug/ml. ~ 75kD band observed in Human Spleen lysates (calculated MW of 76.0kD according to NP_976313.1and NP_976314.1).

Immunogen: C-RTQIDSLYEHIQDC TerminusNP_057318.2; NP_976313.1; NP_976314.1. Homology: Human

Positive Control: Human Spleen lysate

Antibodies; Abs to Coenzyme A

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

55661819

Q9ULC5

75,991 Da

Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Fatty Acid Beta Oxidation Pathway 198865!!Fatty Acid Biosynthesis Pathway 198873!!Fatty Acyl-CoA Biosynthesis Pathway 106115!!Fatty Acid Metabolism Pathway 82935!!Fatty Acid Metabolism Pathway 296!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 160977!!Metabolic Pathways 132956!!Metabolism Of Lipids And Lipoproteins Pathway 160976

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Function: Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage. By similarity. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids. By similarity. It was suggested that it may also stimulate fatty acid oxidation. By similarity. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells. Ref.8 Ref.9 Ref.10. Catalytic activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA. Cofactor: Magnesium. Subcellular location: Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane; Single-pass type III membrane protein. By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein. By similarity Ref.8 Ref.10. Sequence similarities: Belongs to the ATP-dependent AMP-binding enzyme family. Biophysicochemical propertiesKinetic parameters:KM=0.11 uM for palmitic acid (isoform 1 at pH 7.5) Ref.8KM=0.38 uM for palmitic acid (isoform 1 at pH 9.5)KM=0.04 uM for palmitic acid (isoform 3 at pH 7.5)KM=0.15 uM for palmitic acid (isoform 3 at pH 8.5)pH dependence:Optimum pH is 9.5 (isoform 1), 7.5-8.5 (isoform 3). Sequence caution: The sequence BAA85979.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA86054.1 differs from that shown. Reason: Erroneous gene model prediction.

0.1 mg

620 USD