Rabbit anti-human Cytochrome P450 Enzyme CYP3A4 | MyBioSource MBS615441 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti-human Cytochrome P450 Enzyme CYP3A4

catalog :

MBS615441

quantity :

0.1 mL

price :

665 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS615441

products type :

Antibody

products full name :

Rabbit anti-human Cytochrome P450 Enzyme CYP3A4

products short name :

[Cytochrome P450 Enzyme CYP3A4]

products name syn :

[Anti -Cytochrome P450 Enzyme CYP3A4]

other names :

[cytochrome P450 3A4 isoform 1; Cytochrome P450 3A4; cytochrome P450 3A4; OTTHUMP00000210308; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450, family 3, subfamily A, polypeptide 4; 1,8-cineole 2-exo-monooxygenase (EC:1.14.13.157); Albendazole monooxygenase (EC:1.14.13.32); Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase (EC:1.14.13.67); Taurochenodeoxycholate 6-alpha-hydroxylase (EC:1.14.13.97)]

other gene names :

[CYP3A4; CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1; MGC126680; CYP3A3]

uniprot entry name :

CP3A4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Porcine

specificity :

Recognizes human Cytochrome P450 Enzyme CYP3A4 at ~54kD. Species Crossreactivity

purity :

Serum. Serum

form :

Supplied as a liquid. No preservative added.

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:1000-1:2000. Using a microsomal membrane fractions, detects a band at ~54kD although other bands may be present as well. Successfully detects CYP3A4 on LICOR fluorescent westerns. Immunohistochemistry: 1:200-1:400 on 10% formalin fixed, paraffin embedded tissues. Microwave-citric acid antigen recovery is suggested.

other info1 :

Immunogen: Synthetic peptide corresponding to the C-terminal peptide sequence TVSGA from human Cytochrome P450 Enzyme CYP3A4 (KLH). Sequence Homology: CYP3A7, 3A6v4;3A2v1, 3A8, 3A9

other info2 :

Control: Human liver microsomes

products categories :

Antibodies; Abs to Enzymes, Cytochrome

products description :

This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism andsynthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmicreticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4.

ncbi gi num :

13435386

ncbi acc num :

NP_059488.2

ncbi gb acc num :

NM_017460.5

uniprot acc num :

P08684

ncbi mol weight :

57,343 Da

ncbi pathways :

Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Biological Oxidations Pathway (105698); Codeine And Morphine Metabolism Pathway (198831); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fatty Acid Omega Oxidation Pathway (198915)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

uniprot summary :

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1 -hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; Membrane protein, integral; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; Cell surface; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450. Chromosomal Location of Human Ortholog: 7q21.1. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane. Molecular Function: quinine 3-monooxygenase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; albendazole monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxidoreductase activity; oxygen binding; testosterone 6-beta-hydroxylase activity; vitamin D3 25-hydroxylase activity; steroid binding; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; monooxygenase activity. Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; androgen metabolic process; monoterpenoid metabolic process; alkaloid catabolic process; heterocycle metabolic process; lipid metabolic process; drug metabolic process; steroid catabolic process; vitamin D metabolic process

size1 :

0.1 mL

price1 :

665 USD

more info or order :

MyBioSource product webpage