antibody

Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA, Azide & Glycerol Free)

MBS615318

0.5 mL

725 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA, Azide & Glycerol Free)

[Factor XIIIa]

[Anti -Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA, Azide & Glycerol Free)]

[factor XIIIa; Coagulation factor XIII A chain; coagulation factor XIII A chain; TGase; factor XIIIa; fibrinoligase; FSF, A subunit; OTTHUMP00000015990; coagulation factor XIIIa; transglutaminase A chain; transglutaminase. plasma; fibrin stabilizing factor, A subunit; coagulation factor XIII, A polypeptide; protein-glutamine gamma-glutamyltransferase A chain; bA525O21.1 (coagulation factor XIII, A1 polypeptide); coagulation factor XIII, A1 polypeptide; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain]

[F13A1; F13A1; F13A; F13A]

F13A_HUMAN

Polyclonal

IgG

Rabbit

Human

Recognizes human Factor XIIIa, both reduced and non-reduced forms. Does not crossreact with human Factor XIII B-chain or human Factor XII.

Affinity Purified. Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See MBS610527.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 5-10ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 10-20ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Western Blot (WB)

Immunogen: Recombinant protein corresponding to A subunit of coagulation Factor XIII. MW of Antigen: 160kD (Unreduced dimer), 80kD (Reduced monomer). Cellular Localization: Cytoplasmic and nuclear.

Positive Control: Human recombinant Factor XIIIa. Capillary hemangioma, dermatofibroma and placenta.

Antibodies; Abs to Coagulation Factors

Factor XIII is a beta-globulin found in plasma and is composed of two subunits. Factor XIIIa is the catalytic subunit and is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (MW 320kD). Whereas in platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.

412153

P00488

83,267 Da

Common Pathway (106060); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Formation Of Platelet Plug Pathway (106029); Hemostasis Pathway (106028); Platelet Activation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq]

F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family. Protein type: EC 2.3.2.13; Transferase. Chromosomal Location of Human Ortholog: 6p25.3-p24.3. Cellular Component: extracellular region. Molecular Function: protein-glutamine gamma-glutamyltransferase activity; metal ion binding. Biological Process: platelet activation; platelet degranulation; peptide cross-linking; blood coagulation. Disease: Thrombophilia Due To Thrombin Defect; Myocardial Infarction, Susceptibility To; Factor Xiii, A Subunit, Deficiency Of

0.5 mL

725 USD