catalog number :
MBS613921
products short name :
[Brk]
products name syn :
[Anti -Brk]
other names :
[brinker; Brinker; CG9653 gene product from transcript CG9653-RA; CG9653-PA; brinker; brk-PA; small salivary glands-1; brinker]
other gene names :
[brk; brk; Brk; CG9653; Dm brk; DmelCG9653; ssg-1; brinker]
uniprot entry name :
Q9XTN4_DROME
specificity :
Recognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
purity :
Affinity Purified. Purified by immunoaffinity chromatography.
form :
Supplied as a liquid in 0.1M Tris-Glycine, 0.15M Sodium chloride pH 7.4, 0.05% soidum azide.
concentration :
0.5 mg/ml
storage stability :
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
tested application :
Western Blot (WB)
app notes :
Suitable for use in Western Blot. Dilution: Western Blot: 1:500. detected endogenous Brk in SKBR3 cell lysate. Lysate was resolved by electrophoresis, transferred to PVDF, and probed with B2765-01 for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Band observed at ~50kD indicates Rce1. An upper band is thought to be the 73kD ELF-related protein that shares 70% homology with the immunizing peptide.
image1 heading :
Western Blot (WB)
other info1 :
Immunogen: Synthetic peptide corresponding to the N-terminal region of human Brk, conjugated to KLH.
products categories :
Antibodies; Abs to Growth Factors, Cytokines
ncbi acc num :
NP_511069.3
ncbi gb acc num :
NM_078514.3
ncbi mol weight :
77,514 Da