antibody

Pseudomonas aeruginosa Exotoxin A

MBS611247

0.5 mL

380 USD

polyclonal

goat

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

Pseudomonas aeruginosa Exotoxin A

Pseudomonas aeruginosa Exotoxin A

Anti -Pseudomonas aeruginosa Exotoxin A

Polyclonal

IgG

Goat

Recognizes Pseudomonas aeruginosa Exotoxin A.

Serum. Serum

Supplied as a sterile-filtered lyophilized powder. Reconstitute with 500ul sterile ddH2O.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA, Western Blot and Double Immunodiffusion assay. Dilution: Western Blot: 1:200,000. Titer: ELISA: The titer measures at mid-point was 178,000 using 2ug coating of Endotoxin A.

Immunogen: Exotoxin A prepared from P. aeruginosa.

Antibodies; Abs to Infectious Disease Pseudomonas

Exotoxin A is one of the toxic proteins released by pathogenic strains of Pseudomonas aeruginosa. It is secreted as a proenzyme with a molecular weight of 66kD. Like diphtheria toxin, exotoxin A is translocated into susceptible mammalian cells. Covalent alteration of the molecule occurs, rendering it enzymatically active. The intact toxin can be activated 20- to 50-fold in vitro by treatment with urea and dithiothreitol. Exotoxin A catalyzes the transfer of the adenosine diphosphate ribose moiety of oxidized nicotinamide adenine dinucleotide onto elongation factor 2(3): NAD + EF-2 ADP-ribose-EF-2 + nicotinamide + H+. This ADP-ribosylation of EF-2 blocks polypeptide assembly on the surface of the ribosome. As a result, this molecule is cytopathic for a number of cultured cell lines and is toxic to animals. Although exotoxin A and diphtheria toxin have identical enzymatic activity, they exhibit distinct target cell specificities and are immunologically unrelated. In crude preparations or in purified material which has been stored, lower molecular weight peptides with ADP-ribosylating activity may be present. Similarly, after reduction and limited proteolytic cleavage with chymotrypsin, a 26,000 dalton fragment that has enzymatic activity may be separated. This fragment alone is not toxic to intact cells.

0.5 mL

380 USD