RhoGAP, p190B (Rho GTPase-activating protein 5) | MyBioSource MBS610564 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RhoGAP, p190B (Rho GTPase-activating protein 5)

catalog :

MBS610564

quantity :

0.1 mL

price :

550 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot anlaysis of extracts from MCF-7 (human), C2C12 (mouse), and BAEC (bovine) cells, using MBS610564.

product information

catalog number :

MBS610564

products type :

Antibody

products full name :

RhoGAP, p190B (Rho GTPase-activating protein 5)

products short name :

[RhoGAP, p190B]

products name syn :

[Anti -RhoGAP, p190B (Rho GTPase-activating protein 5)]

other names :

[rho GTPase-activating protein 5; Rho GTPase-activating protein 5; rho GTPase-activating protein 5; rho-type GTPase-activating protein 5; Rho GTPase activating protein 5; Rho-type GTPase-activating protein 5; p190-B]

other gene names :

[Arhgap5; Arhgap5; p190B; p190-B; Rhogap5]

uniprot entry name :

RHG05_MOUSE

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Human, Monkey, Mouse, Rat

specificity :

Recognizes endogneous levels of total human RhoGAP protein at 190kD. Species Crossreactivity: monkey, mouse, rat, bovine.

purity :

Affinity Purified. Purified by protein A and peptide affinity chromatography.

form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

storage stability :

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

tested application :

Western Blot (WB)

app notes :

Suitable for use in Western Blot. Dilution: Western Blot: 1:1000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Synthetic peptide corresponding to a region surrounding Lys296 of human p190-B RhoGAP (KLH).

products categories :

Antibodies; Abs to GTPase, Rab, Ras, Rho Proteins

products description :

The Rho family of GTPases, including Rho, Rac and Cdc42, are key regulators of diverse biological processes such as cytoskelatal organization, cell growth and differentiation, transciptional regulation, and cell adhesion/motility (1,2). The activities of these proteins are controlled by GTP binding such that in the GTP-bound state they are active, and in the GDP-bound state they become inactive. Three classes of regulatory proteins control the activity of the GTPases by balancing the levels of GTP/GDP binding: the guanine nucleotide exchange factors (GEFs), which promote the active state by facilitating the exchange of GDP for GTP; the guanine nucleotide dissociation inhibitors GDIs), which sequester the GDP-bound forms and may regulate their intracellular localization; and the GTPase activating proteins (GAPs), which increase GTP hydrolysis to promote the inactive state. The p190 RhoGAP familyconsists of two related proteins, p190-A and p190-B, which are both widely expressed and contain an amino terminal GTPase domain and a carboxy-terminal RhoGAP catalytic domain. Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (3. Phenotypes of these mice are similar to those of CREB knockouts, with reduced cell size, as well as defects in thymus and neural development (3). Cells deficient in p190-B are also defective for adipogenesis, suggesting this pathway is critical for the adipogenesis-myogeneis switch." (4). There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (4-6). Levels of tyrosine phosphorylation are enhanced by Src overexpression (5,6). IGF-1 treament downregulates Rho through phosphorylation and activation of p190-BRhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (4).

ncbi gi num :

86262151

ncbi acc num :

NP_033836.2

ncbi gb acc num :

NM_009706.2

uniprot acc num :

P97393

ncbi mol weight :

172,113 Da

ncbi pathways :

Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Leukocyte Transendothelial Migration Pathway (83280); Leukocyte Transendothelial Migration Pathway (494); Rho GTPase Cycle Pathway (819819); Signal Transduction Pathway (819739); Signaling By Rho GTPases Pathway (819818)

uniprot summary :

ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Protein type: GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs. Biological Process: mammary gland development; positive regulation of mesenchymal cell proliferation; regulation of cell size; positive regulation of cell migration

size1 :

0.1 mL

price1 :

550 USD

more info or order :

MyBioSource product webpage