Clathrin, Heavy Chain | MyBioSource MBS610138 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Clathrin, Heavy Chain

catalog :

MBS610138

quantity :

0.1 mL

price :

550 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot of extrats from SHSY5Y, Neuro2A, PC12 aNone Detected COS cells using MBS610138.

image 2 :

Confocal immunofluorescent analysis of HeLa cells labeled with MBS610138. (green). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).

product information

catalog number :

MBS610138

products type :

Antibody

products full name :

Clathrin, Heavy Chain

products short name :

[Clathrin, Heavy Chain]

products name syn :

[Anti -Clathrin, Heavy Chain]

other names :

[clathrin heavy chain 1; Clathrin heavy chain 1; clathrin heavy chain 1; OTTHUMP00000165784; OTTHUMP00000165785; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); Clathrin heavy chain on chromosome 17]

other gene names :

[CLTC; CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2; KIAA0034; CLH17; CLTCL2; KIAA0034]

uniprot entry name :

CLH1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Human, Monkey, Mouse, Rat

specificity :

Recognizes endogenous levels of total human Clathrin protein at 190 kD.

purity :

Affinity Purified. Purified by immunoaffinity chromatography.

form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

storage stability :

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

tested application :

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Dilution: Western Blot: 1:1000. Immunofluorescence (IF-IC): 1:400

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Synthetic peptide corresponding to residues surrounding Pro1663 of human Clathrin (KLH).

products categories :

Antibodies; Abs to Clathrin

products description :

Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units. (1,2). Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion composed of three clathrin heavy chains and three clathrin light chains. Together with other coat components such as AP2, epsin and EPS15, triskelia are targeted to appropriate membrane fusion sites, where subsequent invagination, cargo protein uptake and vesicle internalization occurs (3,4).

ncbi gi num :

4758012

ncbi acc num :

NP_004850.1

ncbi gb acc num :

NM_004859.3

uniprot acc num :

Q00610

ncbi mol weight :

191,615 Da

ncbi pathways :

Arf6 Trafficking Events Pathway (137954); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Clathrin Derived Vesicle Budding Pathway (119545); EGFR Downregulation Pathway (106342); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

ncbi summary :

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq]

uniprot summary :

CLTC: a major protein component of the cytoplasmic face of coated vesicles and coated pits. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Protein type: Vesicle; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q23.1. Cellular Component: clathrin coat of trans-Golgi network vesicle; clathrin coat; focal adhesion; protein complex; clathrin-coated vesicle; intracellular membrane-bound organelle; cytosol; clathrin coat of coated pit; membrane; cytoplasm; melanosome; plasma membrane; trans-Golgi network membrane; spindle; vesicle. Molecular Function: protein binding; double-stranded RNA binding; structural molecule activity; protein kinase binding; clathrin light chain binding. Biological Process: osteoblast differentiation; mitosis; receptor-mediated endocytosis; intracellular protein transport; receptor internalization; transferrin transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport

size1 :

0.1 mL

price1 :

550 USD

more info or order :

MyBioSource product webpage